Aspartame Assay Kit

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.
Suitable for manual, auto-analyser and microplate formats.

Content/Size SKU Price Qty Stock Status
50 assays (manual) /
500 assays (microplate) / 500 assays (auto-analyser)
K-ASPTM
€158.00
Available
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Advantages

  • Very cost effective
     
  • All reagents stable for > 12 months after preparation
     
  • Only enzymatic kit available
     
  • Measures aspartame and breakdown products (L-aspartate and aspartame acid)
     
  • Very specific
     
  • Very rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials

Principle:
                         (pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH

                      (dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine

                      (glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate

                                 (L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+

Kit size:                             50 assays (manual) / 500 (microplate)
                                           / 500 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                 ~ 5 min
Detection limit:                0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition:       Novel method