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D-Glucose Assay Kit (GOPOD Format)
This test kit contains high purity reagents for the measurement and analysis of D-glucose in cereal extracts and for use in combination with other Megazyme kits.
- All reagents stable for > 12 months after preparation
- Very competitive price (cost per test)
- Simple format
- Standard included
Colourimetric method for the determination of D-Glucose in
foodstuffs, beverages and other materials
(1) D-Glucose + H2O + O2 → D-gluconate + H2O2
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Kit size: 660 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals,
feed, paper and other materials (e.g. biological cultures, samples, etc.)
Widely used and accepted in clinical chemistry and food analysis
Grape and wine analysis: Oenologists to exploit advanced test kits.
Megazyme “advanced” wine test kits general characteristics and validation.
Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris.
In vitro hypoglycemic effects of different insoluble fiber-rich fractions prepared from the peel of Citrus sinensis L. cv. Liucheng
Dietary fibers from mushroom sclerotia: 3. In vitro fermentability using human fecal microflora.
Potential hypoglycaemic effects of insoluble fibres isolated from foxtail millets [Setaria italica (L.) P. Beauvois].
A high-throughput platform for screening milligram quantities of plant biomass for lignocellulose digestibility.
Effects of gamma irradiation on starch digestibility of rice with different resistant starch content.
Anatomical, chemical, and biochemical characterization of cladodes from prickly pear [Opuntia ficus-indica (L.) Mill.].
Structure and digestibility of endosperm water-soluble α-glucans from different sugary maize mutants.
Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: II. Heterotrophic conditions.
In vitro starch digestibility, estimated glycemic index and antioxidant potential of Taro (Colocasia esculenta L. Schott) corm.
Hydrothermal treatment of oleaginous yeast for the recovery of free fatty acids for use in advanced biofuel production.
Effects of heat treatment and moisture contents on interactions between Lauric acid and starch granules.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
- Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
- Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (firstname.lastname@example.org). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
- State the kit lot number being used (this is found on the outside of the kit box).
- State which assay format was used (refer to the relevant page in the kit booklet if necessary).
- State exact details of any modifications to the standard procedure that is provided by Megazyme.
- State the sample type and describe the sample preparation steps if applicable.
The test is best run at pH 7.4. The reagent is buffered at this pH. Using different pH values will affect results, i.e. it may take longer to reach this end point; but the same end-point value should be obtained if not too far away from this pH value.
The test is set up to measure between 10 and 100 micrograms per assay (i.e. 0.1 mL of 0.1 to 1.0 mg/mL). You may prefer to use 3.0 mL of GOPOD reagent mixture plus 1.0 mL of sample; in this case the concentration range in the material being analysed (diluted) should be ~10 to 100 micrograms per mL. The test is linear up to an absorbance of 1.4 (final assay volume of 3.2 mL). If the final volume is 4.0 mL, then linearity will be up to about 1.0 absorbance units.
The reaction is complete after approx. 15 min, and the colour is stable at temperatures of 20-50˚C for at least an extra hour.
1 microgram gives an absorbance of 0.01 if 3 mL of GOPOD are used. If 1 mL of GOPOD is used, 1 microgram gives an absorbance of 0.03 OD.
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
The kit enzymes can be stored at 0-5°C for up to 12 months, so they will be perfectly fine.
The temperature is just for the incubations. It is an end-point assay, so the spectrophotometer does not need to be temperature controlled.
The kit assay will only measure the non-covalently linked monosaccharide.
Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.
To choose a chapter, play the video and select the required chapter from the options on the video display.
Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment
Suitable for manual, auto-analyser and microplate formats.