D-Glucose Assay Kit (GOPOD Format)

This test kit contains high purity reagents for the measurement and analysis of D-glucose in cereal extracts and for use in combination with other Megazyme kits.

Content/Size SKU Price Qty In Stock
660 assays per kit
K-GLUC
$247.00
Available
Add to Cart

Advantages

  • All reagents stable for > 12 months after preparation
     
  • Very competitive price (cost per test)
     
  • Simple format
     
  • Standard included

Colourimetric method for the determination of D-Glucose in
foodstuffs, beverages and other materials

Principle:
                                (glucose oxidase)
(1) D-Glucose + H2O + O2 → D-gluconate + H2O2

                                                                                    (peroxidase)
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
                                                                     quinoneimine + 4H2O

Kit size:                             660 assays
Method:                             Spectrophotometric at 510 nm
Reaction time:                   ~ 20 min
Detection limit:                  100 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals,
feed, paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    
Widely used and accepted in clinical chemistry and food analysis

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris.

Choix. F. J., Bashan, Y., Mendoza, A. & de-Bashan, L. E. (2014). Journal of Biotechnology, 177, 22-34.

In vitro hypoglycemic effects of different insoluble fiber-rich fractions prepared from the peel of Citrus sinensis L. cv. Liucheng

Chau, C. F., Huang, Y. L. & Lee, M. H. (2003). Journal of Agricultural and Food Chemistry, 51(22), 6623-6626.

Dietary fibers from mushroom sclerotia: 3. In vitro fermentability using human fecal microflora.

Wong, K. H., Wong, K. Y., Kwan, H. S. & Cheung, P. C. K. (2005). Journal of Agricultural and Food Chemistry, 53(24), 9407-9412.

Potential hypoglycaemic effects of insoluble fibres isolated from foxtail millets [Setaria italica (L.) P. Beauvois].

Bangoura, M. L., Nsor‐Atindana, J., Zhu, K., Tolno, M. B., Zhou, H. & Wei, P. (2013). International Journal of Food Science & Technology, 48(3), 496-502.

A high-throughput platform for screening milligram quantities of plant biomass for lignocellulose digestibility.

Santoro, N., Cantu, S. L., Tornqvist, C. E., Falbel, T. G., Bolivar, J. L., Patterson, S. E., Pauly, M. & Walton, J. D. (2010). BioEnergy Research, 3(1), 93-102.

Effects of gamma irradiation on starch digestibility of rice with different resistant starch content.

Shu, X., Xu, J., Wang, Y., Rasmussen, S. K. & Wu, D. (2013). International Journal of Food Science & Technology, 48(1), 35-43.

Anatomical, chemical, and biochemical characterization of cladodes from prickly pear [Opuntia ficus-indica (L.) Mill.].

Ginestra, G., Parker, M. L., Bennett, R. N., Robertson, J., Mandalari, G., Narbad, A., Lo Curto, R. B., Bisignano, G., Faulds, C. B. & Waldron, K. W. (2009). Journal of Agricultural and Food Chemistry, 57(21), 10323-10330.

Structure and digestibility of endosperm water-soluble α-glucans from different sugary maize mutants.

Miao, M., Li, R., Jiang, B., Cui, S. W., Lu, K. & Zhang, T. (2014). Food Chemistry, 143, 156-162.

Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: II. Heterotrophic conditions.

Choix, F. J., de-Bashan, L. E. & Bashan, Y. (2012). Enzyme and Microbial Technology, 51(5), 300-309.

In vitro starch digestibility, estimated glycemic index and antioxidant potential of Taro (Colocasia esculenta L. Schott) corm.

Simsek, S. & El, S. N. (2014). Food Chemistry, 168, 257-261.

Hydrothermal treatment of oleaginous yeast for the recovery of free fatty acids for use in advanced biofuel production.

Espinosa-Gonzalez, I., Parashar, A. & Bressler, D. C. (2014). Journal of Biotechnology, 187, 10-15.

Effects of heat treatment and moisture contents on interactions between Lauric acid and starch granules.

Chang, F., He, X., Fu, X., Huang, Q. & Jane, J. L. (2014). Journal of Agricultural and Food Chemistry, 62(31), 7862-7868.
The training video below demonstrates some general principles of wine analysis. DVDs can be requested free of charge by simply adding a note to your online order.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Related Products

High purity reagents for the assay of D-glucose in plant and food products. Can be used in combination with other Megazyme products that require glucose determination.
Product Code:K-GLUHK
Enzymatic UV-method for the measurement of D-fructose and/or D-glucose.
Suitable for manual, auto-analyser and microplate formats.
Product Code:K-FRUGL
Test kit for the measurement and analysis of starch damage in cereal flours.
Product Code:K-SDAM
For the measurement of maltose, sucrose and D-glucose in plant and food products.
Product Code:K-MASUG
For the specific measurement of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.
Product Code:K-MANGL
For the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose,  stachyose and verbascose to D-glucose, D-fructose and D-galactose.
Product Code:K-RAFGL
For the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.
Product Code:K-SUFRG
For the measurement and analysis of sucrose and D-glucose in fruit juice, beverages, honey and food products.
Product Code:K-SUCGL
For the measurement of glucose in the presence of starch or α- or β-D-gluco-oligosaccharides.
Product Code:R-GLC4