Total Dietary Fiber Assay Kit

Total Dietary Fiber Measurement & Analysis. This test kit is suitable for the measurement and analysis of Total Dietary Fiber.

Content/Size SKU Price Qty Stock Status
200 assays per kit
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  • Very competitive price (cost per test)  
  • All reagents stable for > 2 years
  • High purity / standardised enzymes employed
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format


For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds and other materials

       (α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose

(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol

(4) Ash and residual protein determined on DF residues
     and subtracted

Kit size:                              200 assays
Method:                              Hydrolysis / removal of non-dietary
                                            fibre components
Total assay time:              ~ 100 min
Detection limit:                  0.5-100% of sample weight
Application examples:
Food ingredients, food products and other materials
Method recognition:   
AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC
(Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01) and
CODEX (Type I Method)

Total Dietary Fiber Assay Kit, for the measurement and analysis of total, soluble and insoluble dietary fiber according to AOAC and AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81 No. 1, 1998. 

Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.

2. Association of Official Analytical Chemists (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69:370.

3. Association of Official Analytical Chemists (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70:393.

Two separate methods are described in the associated data booklet:



Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).



Based on AACC method 32-05.01 and AOAC Method 985.29.


AACC International (American Associates of Cereal Chemists International)

AACC Method 32-05.01
AACC Method 32-06.01
AACC Method 32-07.01
AACC Method 32-21.01
AACC International is a global, non-profit association working to advance the understanding and knowledge of cereal grain science, and its product development applications. It provides its members with research, practical solutions, methods validation, leadership, and innovative technologies for advancing the grain science community.
AACC International:

Codex Alimentarius

CODEX Method Type I
The Codex Alimentarius Commission, established by FAO and WHO in 1963 develops harmonised international food standards, guidelines and codes of practice to protect the health of the consumers and ensure fair practices in the food trade. The Commission also promotes coordination of all food standards work undertaken by international governmental and non-governmental organisations.
Codex Alimentarius:

AOAC International (Association of Official Analytical Chemists International)

AOAC Method 985.29
AOAC Method 991.42
AOAC Method 991.43
AOAC Method 993.19
AOAC International is a global, independent, non-profit association which provides an analytical platform for finding appropriate science-based solutions for its members as analytical needs arise. AOAC’s primary pursuit is the development of globally accepted standards. They are committed to testing and validating analytical methods by scientific studies and collaboration between members.
AOAC International:

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