Synonyms: 4-Methylumbelliferyl β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside
Molecular Formula: C28H38O18
Molecular Weight: 662.6
Purity: > 90% (contains ~ 5% 4MU-α-anomer)
High purity 4-Methylumbelliferyl-β-(1,3:1,4)-glucotrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a fluorimetric substrate for the detection or measurement of lichenase or mixed linkage β-glucanase (endo-1,3:1,4-β-D-glucanase) activity. As this substrate can also be hydrolysed by exo-acting β-glucanase/β-glucosidase enzymes, it is recommended only for the assay of pure enzyme solutions. The data sheet for the analogous UV-based substrate, 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotrioside (cat. no. O-CNPBG3), describes general assay conditions although the substrate concentration can be significantly reduced for this fluorimetric version of the assay. Please note that a fluorimeter is required.
Novel approaches to the automated assay of β-glucanase and lichenase activity.