High purity dyed, soluble AZ-Rhamnogalacturonan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. 

Substrate for the specific assay of endo-rhamnogalacturonanase.

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Product Code
2 grams

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Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module.

Brown, I. E., Mallen, M. H., Charnock, S. J., Davies, G. J. & Black, G. W. (2001). Biochemical Journal, 355(1), 155-165.

Characterization of thermophilic halotolerant Aeribacillus pallidus TD1 from Tao dam hot spring, Thailand.

Yasawong, M., Areekit, S., Pakpitchareon, A., Santiwatanakul, S. & Chansiri, K. (2011). International Journal of Molecular Sciences, 12(8), 5294-5303.

Degradation of cell wall materials from sweetpotato, cassava, and potato by a bacterial protopectinase and terminal sugar analysis of the resulting solubilized products.

Salvador, L. D., Suganuma, T., Kitahara, K., Fukushige, Y. & Tanoue, H. (2002). Journal of Bioscience and Bioengineering, 93(1), 64-72.

Discovery of pectin-degrading enzymes and directed evolution of a novel pectate lyase for processing cotton fabric.

Solbak, A., Richardson, T. H., McCann, R. T., Kline, K. A., Bartnek, F., Tomlinson, G., Tan, X., Parra-Gessert, L., Frey, G. J., Podar, M., Luginbu¨ hl, P., Gray, K. A., Mathur, E. J., Robertson, D. E., Burk, M. J., Hazlewood, G. P., Short, J. M. & Kerovuo, J. (2005). Journal of Biological Chemistry, 280(10), 9431-9438.

Negative subtraction hybridization: an efficient method to isolate large numbers of condition-specific cDNAs.

Ray, A., Macwana, S., Ayoubi, P., Hall, L. T., Prade, R. & Mort, A. J. (2004). BMC Genomics, 5(1), 22.
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Principle of the Assay Procedure
Chapter 2: Substrate & Kit Description
Chapter 3: Dissolution of Azo-CM-Cellulose
Chapter 4: Precipitant Solution
Chapter 5: Preparation of Buffer Solution
Chapter 6: Assay Procedure
Chapter 7: Calculation

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