Acetaldehyde Assay Kit

The Acetaldehyde test kit is a simple, reliable and accurate method for the measurement and analysis of acetaldehyde in beverages and foodstuffs.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

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Product Code
Content/size
Stock
Price
Qty
K-ACHYD
50 assays (manual) / 500 assays (microplate)
/ 500 assays (auto-analyser)
$164.00

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UV-method for the determination of Acetaldehyde in foodstuffs, beverages and other materials

Principle:
                                   (aldehyde dehydrogenase)
(1) Acetaldehyde + NAD+ + H2O    →    acetic acid + NADH + H+

Kit size:                             50 assays (manual) / 500 (microplate)
                                           / 500 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 4 min
Detection limit:                 0.18 mg/L
Application examples:
Wine, champagne, beer, liqueurs, brandy, dairy products (e.g. yogurt),
bread, fruit juices, soft drinks, cocoa, vegetable and fruit products,
coffee, and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by MEBAK

Advantages

  • No wasted aldehyde dehydrogenase solution (stable suspension supplied)
     
  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years after preparation
     
  • Simple format
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Extended cofactors stability
     
  • Suitable for manual, microplate and auto-analyser formats

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Taraxerone enhances alcohol oxidation via increases of alcohol dehyderogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities and gene expressions.

Sung, C. K., Kim, S. M., Oh, C. J., Yang, S. A., Han, B. H. & Mo, E. K. (2012). Food and Chemical Toxicology, 50(7), 2508-2514.

Effects of Chitooligosaccharide Lactate Salt on Activity of Acetaldehyde Dehydrogenase.

Cho, S. Y., Yun, J. W., Park, P. J., Sohn, J. H., Seo, D. B., Lim, K. M., Kim, W. G. & Lee, S. J. (2010). Journal of Medicinal Food, 13(5), 1061-1068.

Exploitation of the semi‐homothallic life cycle of Saccharomyces cerevisiae for the development of breeding strategies.

Zara, G., Mannazzu, I., Sanna, M. L., Orro, D., Farris, G. A. & Budroni, M. (2008). FEMS Yeast Research, 8(7), 1147-1154.

Potential of the waste from beer fermentation broth for bio-ethanol production without any additional enzyme, microbial cells and carbohydrates.

Ha, J. H., Shah, N., Ul-Islam, M. & Park, J. K. (2011). Enzyme and Microbial Technology, 49(3), 298-304.

Functional improvement of Saccharomyces cerevisiae to reduce volatile acidity in wine.

Luo, Z., Walkey, C. J., Madilao, L. L., Measday, V. & Vuuren, H. J. (2013). FEMS Yeast Research, 13(5), 485-494.

Alcohol Metabolism in Human Cells Causes DNA Damage and Activates the Fanconi Anemia–Breast Cancer Susceptibility (FA‐BRCA) DNA Damage Response Network.

Abraham, J., Balbo, S., Crabb, D. & Brooks, P. J. (2011). Alcoholism: Clinical and Experimental Research, 35(12), 2113-2120.

Effect of thermal processing during yogurt production upon the detection of staphylococcal enterotoxin B.

Principato, M., Boyle, T., Njoroge, J., Jones, R. L. & O'Donnell, M. (2009). Journal of Food Protection®,72(10), 2212-2216.
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Auto-analyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs