Acetic Acid Assay Kit (Acetate Kinase Manual Format) 

The Acetic Acid (Acetate Kinase Manual Format) test kit is suitable for the measurement and analysis of acetic acid in food and beverages.

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.

Suitable for manual and microplate formats.

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Product Code
Content/size
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Price
Qty
K-ACETRM
72 assays (manual) / 720 assays (microplate)
$236.00

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Manual format UV-method for the determination of Acetic Acid
in foodstuffs, beverages and other materials


Principle:
                         (acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP

                          (phosphotransacetylase)
(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi

           (pyruvate kinase)
(3) ADP + PEP → ATP + pyruvate

                       (D-lactate dehydrogenase)
(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+

Kit size:                            72 assays (manual) / 720 (microplate)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 4 min
Detection limit:                 0.254 mg/L
Application examples: 
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, 
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    Improved method

Advantages

  • Improved assay format (only two absorbance readings required)
     
  • All reagents stable for > 2 years after preparation
     
  • PVP incorporated to prevent tannin inhibition
     
  • Very rapid reaction (~ 4 min)
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Very competitive price (cost per test)
     
  • Suitable for manual and microplate formats

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

The metabolic potential of Escherichia coli BL21 in defined and rich medium.

Li, Z., Nimtz, M. & Rinas, U. (2014). Microbial Cell Factories, 13(1), 45.

Comparative transcriptomic profile analysis of fed-batch cultures expressing different recombinant proteins in Escherichia coli.

Sharma, A. K., Mahalik, S., Ghosh, C., Singh, A. B. & Mukherjee, K. J. (2011). AMB Express, 1(1), 33.

Organic carbon supplementation of sterilized municipal wastewater is essential for heterotrophic growth and removing ammonium by the microalga Chlorella Vulgaris.

Perez‐Garcia, O., Bashan, Y. & Esther Puente, M. (2011). Journal of Phycology, 47(1), 190-199.

Large-scale metabolome analysis and quantitative integration with genomics and proteomics data in Mycoplasma pneumonia.

Maier, T., Marcos, J., Wodke, J. A. H., Paetzold, B., Liebeke, M., Gutiérrez-Gallego, R. & Serrano, L. (2013). Molecular BioSystems, 9(7), 1743-1755.

Decline in mammary translational capacity during intravenous glucose infusion into lactating dairy cows.

Curtis, R. V., Kim, J. J. M., Bajramaj, D. L., Doelman, J., Osborne, V. R. & Cant, J. P. (2014). Journal of Dairy Science, 97(1), 430-438.

A new family of carbohydrate esterases is represented by a GDSL hydrolase/acetylxylan esterase from Geobacillus stearothermophilus.

Alalouf, O., Balazs, Y., Volkinshtein, M., Grimpel, Y., Shoham, G. & Shoham, Y. (2011). Journal of Biological Chemistry, 286(49), 41993-42001.

Microbial carbohydrate esterases deacetylating plant polysaccharides.

Biely, P. (2012). Biotechnology Advances, 30(6), 1575-1588.

Assay for peptidoglycan O-acetyltransferase: A potential new antibacterial target.

Moynihan, P. J. & Clarke, A. J. (2013). Analytical Biochemistry, 439(2), 73-79.

Discovery of diversity in xylan biosynthetic genes by transcriptional profiling of a heteroxylan containing mucilaginous tissue.

Jensen, J. K., Johnson, N. & Wilkerson, C. G. (2013). Frontiers in Plant Science, 4(183).

Structural changes and cellular localization of resuscitation-promoting factor in environmental isolates of Micrococcus luteus.

Koltunov, V., Greenblatt, C. L., Goncharenko, A. V., Demina, G. R., Klein, B. Y., Young, M. & Kaprelyants, A. S. (2010). Microbial Ecology, 59(2), 296-310.
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Auto-analyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs