Acetic Acid Assay Kit (Acetate Kinase Manual Format) 

The Acetic Acid (Acetate Kinase Manual Format) test kit is suitable for the measurement and analysis of acetic acid in food and beverages.

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.

Suitable for manual and microplate formats.

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Product Code
72 assays (manual) / 720 assays (microplate)

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Manual format UV-method for the determination of Acetic Acid
in foodstuffs, beverages and other materials

                         (acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP

(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi

           (pyruvate kinase)
(3) ADP + PEP → ATP + pyruvate

                       (D-lactate dehydrogenase)
(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+

Kit size:                            72 assays (manual) / 720 (microplate)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 4 min
Detection limit:                 0.254 mg/L
Application examples: 
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, 
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    Improved method


  • Improved assay format (only two absorbance readings required)
  • All reagents stable for > 2 years after preparation
  • PVP incorporated to prevent tannin inhibition
  • Very rapid reaction (~ 4 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Very competitive price (cost per test)
  • Suitable for manual and microplate formats

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