Acetylxylan esterase (Orpinomyces sp.

High purity recombinant Acetylxylan esterase (Orpinomyces sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: CE6
CAS: 188959-24-2 / 9000-82-2

acetylxylan esterase

Recombinant. From Orpinomyces sp.
In 3.2 M ammonium sulphate.
E-AXEAO-1KU: Supplied at ~ 1,000 U/mL.
E-AXEAO-3KU: Supplied at ~ 1,000 U/mL.

Specific activity:
~ 36 U/mg (40oC, pH 6.7 on 4-nitrophenyl acetate).

Stability: Minimum 1 year at 4oC. Check vial for details.

Data booklets for each pack size are located in the Documentation tab.

Product Code
1,000 units
3,000 units

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Acetylxylan esterase (Orpinomyces sp.)

CAZy Family: CE6
CAS: 188959-24-2 / 9000-82-2

acetylxylan esterase

In 3.2 M ammonium sulphate.

Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 36 U/mg (40oC, pH 6.7 on 4-nitrophenyl acetate).

Unit definition:
One Unit of acetylxylan esterase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl acetate in sodium phosphate buffer (100 mM), pH 6.7 at 40oC.

Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, α-napthyl acetate and 4-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.

Applications established in carbohydrate research and biofuel industries.

Positional specifity of acetylxylan esterases on natural polysaccharide: An NMR study.

Uhliariková, I., Vršanská, M., McCleary, B. V. & Biely, P. (2013). Biochimica et Biophysica Acta (BBA), 1830(6), 3365–3372.

CE16 on acetyl-4-O-methylglucuronoxylan and acetyl-galactoglucomannan.

Mai-Gisondi, G., Maaheimo, H., Chong, S. L., Hinz, S., Tenkanen, M. & Master, E. (2017). Biochimica et Biophysica Acta (BBA)-General Subjects, 1861(9), 2398-2405.

Enzymatic Systems for Cellulose Acetate Degradation.

Haske-Cornelius, O., Pellis, A., Tegl, G., Wurz, S., Saake, B., Ludwig, R., Sebastian, A., Gibson S. Nyanhongo, G. S. & Guebitz, G. M. (2017). Catalysts, 7(10), 287.

Action of different types of endoxylanases on eucalyptus xylan in situ.

Puchart, V., Fraňová, L., Krogh, K. B. M., Hoff, T. & Biely, P. (2018). Applied Microbiology and Biotechnology, 1-12.

Comprehensive evaluation of combining hydrothermal pretreatment (autohydrolysis) with enzymatic hydrolysis for efficient release of monosaccharides and ferulic acid from corn bran.

iang, K., Li, L., Long, L. & Ding, S. (2018). Industrial Crops & Products, 113, 348-357.

A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans.

Razeq, F. M., Jurak, E., Stogios, P. J., Yan, R., Tenkanen, M., Kabel, M. A., Wang, W. & Master, E. R. (2018). Biotechnology for Biofuels, 11(1), 74.

Structural characterization of hemicellulose released from corn cob in continuous flow type hydrothermal reactor.

Arai, T., Biely, P., Uhliariková, I., Sato, N., Makishima, S., Mizuno, M., Nozaki, K., Kaneko, S. & Amano, Y. (2018). Journal of Bioscience and Bioengineering, In Press.