Acetylxylan esterase (Orpinomyces sp.

High purity recombinant Acetylxylan esterase (Orpinomyces sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.1.1.72
CAZy Family: CE6
CAS: 188959-24-2 / 9000-82-2

acetylxylan esterase

Recombinant. From Orpinomyces sp.
In 3.2 M ammonium sulphate.
E-AXEAO-1KU: Supplied at ~ 1,000 U/mL.
E-AXEAO-3KU: Supplied at ~ 1,000 U/mL.

Specific activity:
~ 290 U/mg (40oC, pH 6.7 on 4-nitrophenyl acetate).

Stability: > 2 years at 4oC.

Data booklets for each pack size are located in the Documentation tab.

Product Code
Content/Size
Stock
Price
Qty
E-AXEAO-1KU
1,000 units
$121.00
E-AXEAO-3KU
3,000 units
$235.00

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DESCRIPTION

Acetylxylan esterase (Orpinomyces sp.)

EC 3.1.1.72
CAZy Family: CE6
CAS: 188959-24-2 / 9000-82-2

Synonyms
acetylxylan esterase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 2 years at 4oC.

Specific activity:
~ 290 U/mg (40oC, pH 6.7 on 4-nitrophenyl acetate).

Unit definition:
One Unit of acetylxylan esterase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl acetate in sodium phosphate buffer (100 mM), pH 6.7 at 40oC.

Specificity:
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, α-napthyl acetate and 4-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.

Applications:
Applications established in carbohydrate research and biofuel industries.

Positional specifity of acetylxylan esterases on natural polysaccharide: An NMR study.

Uhliariková, I., Vršanská, M., McCleary, B. V. & Biely, P. (2013). Biochimica et Biophysica Acta (BBA), 1830(6), 3365–3372.

CE16 on acetyl-4-O-methylglucuronoxylan and acetyl-galactoglucomannan.

Mai-Gisondi, G., Maaheimo, H., Chong, S. L., Hinz, S., Tenkanen, M. & Master, E. (2017). Biochimica et Biophysica Acta (BBA)-General Subjects, In press.