Aldopentauronic acid (internal substitution, borohydride reduced)
Molecular Formula: C27H46O23
Molecular Weight: 738.6
Purity: > 50% (TLC)
23-(4-O-methyl-D-glucuronyl)-α-D-xylotetraitol (XUXX-R) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Prepared by the controlled enzymatic hydrolysis of 4-O-methyl-glucuronoxylan, followed by chromatographic removal of neutral oligomers and borohydride reduction. Note that an unidentified aldouronic acid (suspected to be a higher oligomer) is also present along with the title compound in ratio of approximately 1:1. The unidentified impurity is not hydrolysed by GH67 or GH115 α-glucuronidases or GH43 β-xylosidase. XUXX-R is a selective substrate for GH115 α-glucuronidases and is not hydrolysed by the GH67 family.
Borohydride reduced oligosaccharides are particularly useful as substrates in reducing sugar assays (e.g. Nelson Somogyi) as they do not give rise to the large background / blank value observed for the analogous non-reduced native oligosaccharides.
Identification, heterologous expression and characterization of a novel glycoside hydrolase family 30 xylanase from the fungus Penicillium purpurogenum.