Aldotriouronic acid (terminal substitution, borohydride reduced)
Molecular Formula: C22H38O19
Molecular Weight: 606.5
Purity: > 90% (TLC)
High purity 23-(4-O-methyl-D-glucuronyl)-α-D-xylotriitol for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Prepared by the controlled enzymatic hydrolysis of 4-O-methyl-glucuronoxylan, followed by chromatographic removal of neutral oligomers and borohydride reduction. This compound is a substrate for the assay of both GH67 and GH115 α-glucuronidases, although the rate of hydrolysis by the GH67 family is significantly higher than that for the GH115 family.
Borohydride reduced oligosaccharides are particularly useful as substrates in reducing sugar assays (e.g. Nelson Somogyi) as they do not give rise to the large background / blank value observed for the analogous non-reduced native oligosaccharides.
Isolation of Carignan and Merlot red wine oligosaccharides and their characterization by ESI-MS.