Alginate lyase (Sphingomonas sp.) 

High purity recombinant Alginate lyase (Sphingomonas sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 4.2.2.3
CAZy Family: PL7
CAS: 86922-62-5; 9024-15-1

poly(beta-D-mannuronate) lyase; poly[(1,4)-beta-D-mannuronide] lyase

Recombinant. From Sphingomonas sp.
In 3.2 M ammonium sulphate.
Supplied at ~ 2,500 U/mL. 

Specific activity:
~ 120 U/mg (40oC, pH 7.2 on sodium alginate).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-ALGLS
5,000 Units
$185.00

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DESCRIPTION

Alginate lyase (Sphingomonas sp.)

EC 4.2.2.3
CAZy Family: PL7
CAS: 86922-62-5; 9024-15-1

Synonyms:
poly(beta-D-mannuronate) lyase; poly[(1,4)-beta-D-mannuronide] lyase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 120 U/mg (40oC, pH 7.2 on sodium alginate).

Unit definition:
One Unit of alginate lyase activity is defined as the amount of enzyme required to produce an increase in absorbance of 1.0 per minute at 235 nm in Tris.HCl buffer (100mM), pH 7.2 at 40oC.

Specificity:
endo-acting β-elimination cleavage of the polysaccharide, alginate.

Applications:
Utilised in research, biochemical assays and in vitro binding studies and as a biocatalyst for saccharification of alginate.

Separation and Identification of Individual Alginate Oligosaccharides in the Feces of Alginate-Fed Pigs.

Jonathan, M. C., Bosch, G., Schols, H. A. & Gruppen, H. (2013). Journal of Agricultural and Food Chemistry, 61(3), 553-560.