α-Glucosidase (Bacillus stearothermophilus) (Recombinant) 

High purity recombinant α-Glucosidase (Bacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.20
CAZy Family: GH13
CAS: 9001-42-7

alpha-glucosidase; alpha-D-glucoside glucohydrolase

Recombinant. From Bacillus stearothermophilus.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,500 U/mL. 

Specific activity:
~ 280 U/mg (60oC, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside);
~ 135 U/mg (40oC, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-TSAGS
3,000 Units at 40oC;
~ 6,000 Units at 60oC
$185.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

α-Glucosidase (Bacillus stearothermophilus)

EC 3.2.1.20
CAZy Family: GH13
CAS: 9001-42-7

Synonyms:
alpha-glucosidase; alpha-D-glucoside glucohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 280 U/mg (60oC, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside);
~ 135 U/mg (40oC, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside).

Unit definition:
One Unit of α-D-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl α-D-glucopyranoside (5 mM), in sodium phosphate buffer (100 mM), pH 6.5 at 40oC.

Specificity:
Hydrolysis of terminal, non-reducing α-1,4-linked D-glucose residues with release of D-glucose.

Applications:
Applications in carbohydrate and biofuels research and diagnostic and analytical procedures.

Highly hydrolytic reuteransucrase from probiotic Lactobacillus reuteri strain ATCC 55730.

Kralj, S., Stripling, E., Sanders, P., van Geel-Schutten, G. H. & Dijkhuizen, L. (2005). Applied and Environmental Microbiology, 71(7), 3942-3950.