α-Glucosidase (transglucosidase) (Aspergillus niger

High purity α-Glucosidase (transglucosidase) (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.20
CAZy Family: GH31
CAS: 9001-42-7

alpha-glucosidase; alpha-D-glucoside glucohydrolase

Highly purified. From Aspergillus niger.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,000 U/mL. 

Specific activity
~ 70 U/mg (40oC, pH 4.5 on maltose);
~ 2.5 U/mg (40oC, pH 4.5 on phenyl α-glucoside).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-TRNGL
2,000 Units
$182.00

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DESCRIPTION

α-Glucosidase (transglucosidase) (Aspergillus niger)

EC 3.2.1.20
CAZy Family: GH31
CAS: 9001-42-7

Synonyms:
alpha-glucosidase; alpha-D-glucoside glucohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 70 U/mg (40oC, pH 4.5 on maltose);
~ 2.5 U/mg (40oC, pH 4.5 on phenyl α-glucoside).

Unit definition:
One Unit of α-Glucosidase activity is defined as the amount of enzyme required to release one μmole of glucose per minute from maltose (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.

Specificity:
Hydrolysis of terminal, non-reducing α-1,4-linked D-glucose residues with release of D-glucose.

Applications:
Applications in carbohydrate and biofuels research and diagnostic and analytical procedures.

Purification, properties, and industrial significance of transglucosidase from Aspergillus niger.

McCleary, B. V., Gibson, T. S., Sheehan, H., Casey, A., Horgan, L. & O’Flaherty, J. (1989). Carbohydrate Research, 185(1), 147-162.

Weissella confusa Cab3 dextransucrase: Properties and in vitro synthesis of dextran and glucooligosaccharides.

Shukla, S., Shi, Q., Maina, N. H., Juvonen, M., Tenkanen, M. & Goyal, A. (2014). Carbohydrate Polymers, 101, 554-564.

Structural analysis of enzyme-resistant isomaltooligosaccharides reveals the elongation of α-(1→ 3)-linked branches in Weissella confusa dextran.

Maina, N. H., Virkki, L., Pynnönen, H., Maaheimo, H. & Tenkanen, M. (2011). Biomacromolecules, 12(2), 409-418.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.