α-Glucuronidase Assay Kit
The α-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of α-D-glucuronidase in various enzyme preparations. The kit contains a pure aldotriouronic acid substrate and an α-D-glucuronidase (GH67) control enzyme.
Suitable for manual and microplate formats.
New, improved substrate.
This kit contains highly purified, borohydride reduced aldotriouronic acid with a terminal α-D-glucuronic acid substitution, which is an excellent substrate for α-D-glucuronidases from GH67. This substrate can be also used for the measurement of α-D-glucuronidases from GH115, however the rate of hydrolysis compared to GH67 is reduced. Previously this kit contained a mixture of borohydride reduced aldouronic acids (tri:tetra:penta).
UV-method for the measurement of α-D-Glucuronidase in
various enzyme preparations
(1) Aldouronic acid (NaBH4 reduced) + H2O →
β-(1,4)-D-xylo-oligosaccharides + D-glucuronic acid
(uronate dehydrogenase; UDH)
(2) D-Glucuronic acid + NAD+ + H2O → D-glucarate + NADH + H+
Kit size: 50 assays (manual) / 200 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 25 min
Detection limit: 17 mU/mL
Enzyme preparations and other materials
Method recognition: Novel method
- Very competitive price (cost per test)
- All reagents stable for > 2 years as supplied
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
Evidence that GH115 α-glucuronidase activity, which is required to degrade plant biomass, is dependent on conformational flexibility.
Expression and Characterization of Glycosyl Hydrolase Family 115 α-Glucuronidase from Scheffersomyces stipitis.
Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.FAQs