α-Glucuronidase Assay Kit

The α-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of α-D-glucuronidase in various enzyme preparations. The kit contains a pure aldotriouronic acid substrate and an α-D-glucuronidase (GH67) control enzyme.

Suitable for manual and microplate formats.

New, improved substrate.

This kit contains highly purified, borohydride reduced aldotriouronic acid with a terminal α-D-glucuronic acid substitution, which is an excellent substrate for α-D-glucuronidases from GH67. This substrate can be also used for the measurement of α-D-glucuronidases from GH115, however the rate of hydrolysis compared to GH67 is reduced. Previously this kit contained a mixture of borohydride reduced aldouronic acids (tri:tetra:penta).

Product Code
Content/size
Stock
Price
Qty
K-AGLUA
50 assays (manual) / 200 assays (microplate)
$258.00

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UV-method for the measurement of α-D-Glucuronidase in
various enzyme preparations

Principle:
                                                           (α-D-glucuronidase)
(1) Aldouronic acid (NaBH4 reduced) + H2O →
                       β-(1,4)-D-xylo-oligosaccharides + D-glucuronic acid


                                         (uronate dehydrogenase; UDH)
(2) D-Glucuronic acid + NAD+ + H2O → D-glucarate + NADH + H+

Kit size:                             50 assays (manual) / 200 (microplate)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 25 min
Detection limit:                 17 mU/mL
Application examples:
Enzyme preparations and other materials
Method recognition:     Novel method

Advantages

  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years as supplied
     
  • Simple format
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Evidence that GH115 α-glucuronidase activity, which is required to degrade plant biomass, is dependent on conformational flexibility.

Rogowski, A., Baslé, A., Farinas, C. S., Solovyova, A., Mortimer, J. C., Dupree, P., Gilbert, H. J. & Bolam, D. N. (2014). Journal of Biological Chemistry, 289(1), 53-64.

Expression and Characterization of Glycosyl Hydrolase Family 115 α-Glucuronidase from Scheffersomyces stipitis.

Bajwa, P. K., Harrington, S., Dashtban, M. & Lee, H. (2016). Industrial Biotechnology, 12(2), 98-104.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs