α-Glucuronidase (Geobacillus stearothermophilus) (Recombinant)

High purity recombinant α-Glucuronidase (Geobacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.139
CAZy Family: GH67
CAS: 37259-81-7

alpha-glucuronidase; alpha-D-glucosiduronate glucuronohydrolase

Recombinant. From Geobacillus stearothermophilus.
In 3.2 M ammonium sulphate.
Supplied at ~ 100 U/mL.

Specific activity:
~ 7 U/mg (70oC, pH 7.0 on aldouronic acid mixture; tri:tetra:penta);
~ 2 U/mg (40oC, pH 7.0 on aldouronic acid mixture; tri:tetra:penta)
.

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-AGUBS
200 Units at 70oC
$175.00

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DESCRIPTION

α-glucuronidase (Geobacillus stearothermophilus)

EC 3.2.1.139
CAZy Family: GH67
CAS: 37259-81-7

Synonyms:
alpha-glucuronidase; alpha-D-glucosiduronate glucuronohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 7 U/mg (70oC, pH 7.0 on aldouronic acid mixture; tri:tetra:penta);
~ 2 U/mg (40oC, pH 7.0 on aldouronic acid mixture; tri:tetra:penta).

Unit definition:
One Unit of α-D-glucuronidase activity is defined as the amount of enzyme required to release one µmole of α-D-glucuronic acid per minute from aldouronic acid (tri:tetra:penta) in MOPS buffer (100 mM), pH 7.0 at 70oC. 

Specificity:
Hydrolysis of the α-1,2 glycosidic bond between D-glucuronic acid or its ether 4-O-methyl-D-glucuronic acid from the terminal non-reducing D-xylose residues of xylo-oligosaccharides (aldo-uronic acids) and xylan.

Applications:
Applications in carbohydrate research, the paper pulp industry and preparation of xylans for pharmaceutical and cosmetic formulations.

The reducing end sequence of wheat endosperm cell wall arabinoxylans.

Ratnayake, S., Beahan, C. T., Callahan, D. L. & Bacic, A. (2014). Carbohydrate Research, 386, 23-32.