α-Rhamnosidase (prokaryote) 

High purity recombinant α-Rhamnosidase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.40
CAZy family: GH78
CAS: 37288-35-0

alpha-L-rhamnosidase; alpha-L-rhamnoside rhamnohydrolase

Recombinant. From a prokaryotic source. 
In 3.2 M ammonium sulphate.
Supplied at ~ 1,500 U/mL. 

Specific activity:
~ 190 U/mg (50oC, pH 6.5 on p-nitrophenyl-α-L-rhamnoside).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-RHAMS
3,000 Units at 50oC
$185.00

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DESCRIPTION

α-Rhamnosidase (prokaryote)

EC 3.2.1.40
CAZy Family: GH78

CAS: 37288-35-0

Synonyms:
alpha-L-rhamnosidase; alpha-L-rhamnoside rhamnohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 190 U/mg (50oC, pH 6.5 on p-nitrophenyl-α-L-rhamnoside).

Unit definition:
One Unit of α-L-rhamnosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-rhamnoside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 50oC.

Specificity:
Hydrolysis of terminal non-reducing α-L-rhamnose residues in α-L-rhamnosides.

Applications:
Applications in carbohydrate and biofuels research.

Effect of high hydrostatic pressure treatment on isoquercetin production from rutin by commercial α-L-rhamnosidase.

Kim, D. Y., Yeom, S. J., Park, C. S. & Kim, Y. S. (2016). Biotechnology Letters, 38(10), 1775-1780.

Characterisation of the mucilage polysaccharides from Dioscorea opposita Thunb. with enzymatic hydrolysis.

Ma, F., Wang, D., Zhang, Y., Li, M., Qing, W., Tikkanen-Kaukanen, C., Liu, X. & Bell, A. E. (2018). Food Chemistry, 245, 13-21.