Amyloglucosidase (Aspergillus niger) Glycerol Free

High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

EC 3.2.1.3 
CAZy Family: GH15
CAS: 9032-08-0 

glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase

From Aspergillus niger.
In buffered solution.

This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Megazyme Total Starch test method.

The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method). 

Specific activity:
~ 36 U/mg (40oC, pH 4.5 on soluble starch);
~ 400 U/mL on p-nitrophenyl β-maltoside;
~ 6,520 U/mL on soluble starch.

Stability: > 4 years 4oC.

Data booklets for each pack size are located in the Documentation tab.

View Megazyme’s latest Guide for Dietary Fiber Analysis.

Product Code
Content/Size
Stock
Price
Qty
E-AMGDFNG-20ML
20ML
$188.00
E-AMGDFNG-50ML
50ML
$376.00

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DESCRIPTION

Amyloglucosidase (Aspergillus niger) Glycerol Free

EC 3.2.1.3 
CAZy Family: GH15
CAS: 9032-08-0 

Synonyms:
glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase

Form:
Buffered solution

Stability:
> 4 years 4oC

Specific activity:
~ 36 U/mg (40oC, pH 4.5 on soluble starch);
~ 400 U/mL on p-nitrophenyl β-maltoside;
~ 6,520 U/mL on soluble starch.

Unit definition:
One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 at 40oC.

Specificity:
Hydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose.

Applications:
This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Megazyme Total Starch test method.

The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).