Amyloglucosidase (Rhizopus sp.) 

High purity Amyloglucosidase (Rhizopus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: GH15
CAS: 9032-08-0

glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase 

Ultra High purity. From Rhizopus sp.
Freeze-dried powder.

Specific activity:
~ 35 U/mg (40oC, pH 4.5 on soluble starch).

Stability: Minimum 1 year at < -10oC. Check vial for details.

Product Code
5,000 Units

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Amyloglucosidase (Rhizopus sp.)

CAZy Family: GH15
CAS: 9032-08-0

glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase

Freeze-dried powder.

Minimum 1 year at < -10oC. Check vial for details.

Specific activity:
~ 35 U/mg (40oC, pH 4.5 on soluble starch).

Unit definition:.
One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC.

Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.

Applications for the measurement of starch and dietary fiber in the food and feeds industry.

Hydrolysis of α-D-glucans and α-D-gluco-oligosaccharides by cladosporium resinae glucoamylases.

McCleary, B. V. & Anderson, M. A. (1980). Carbohydrate Research, 86(1), 77-96.

Measurement of amyloglucosidase using P-nitrophenyl β-maltoside as substrate.

McCleary, B. V., Bouhet, F. & Driguez, H. (1991). Biotechnology Techniques, 5(4), 255-258.

Enzyme purity and activity in fibre determinations.

McCleary, B. V. (1999). Cereal Foods World, 44(8), 590-596.

Importance of enzyme purity and activity in the measurement of total dietary fibre and dietary fibre components.

McCleary, B. V. (2000). Journal of AOAC International, 83(4), 997-1005.

Characterisation of the substituent distribution in hydroxypropylated potato amylopectin starch.

Richardson, S., Nilsson, G. S., Bergquist, K. E., Gorton, L. & Mischnick, P. (2000). Carbohydrate Research, 328(3), 365-373.

Method for the direct determination of available carbohydrates in low-carbohydrate products using high-performance anion exchange chromatography.

Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W. & Ragan, R. (2010). Journal of AOAC International, 93(6), 1897-1904.

Spatial division of phosphoenolpyruvate carboxylase and nitrate reductase activity and its regulation by cytokinins in CAM-induced leaves of Guzmania monostachia (Bromeliaceae).

Pereira, P. N., Purgatto, E. & Mercier, H. (2013). Journal of Plant Physiology, 170(12), 1067-1074.

Nitrogen metabolism in leaves of a tank epiphytic bromeliad: Characterization of a spatial and functional division.

Takahashi, C. A. & Mercier, H. (2011). Journal of Plant Physiology, 168(11), 1208-1216.

High-performance anion-exchange chromatography–electrospray mass spectrometry for investigation of the substituent distribution in hydroxypropylated potato amylopectin starch.

Richardson, S., Cohen, A. & Gorton, L. (2001). Journal of Chromatography A, 917(1), 111-121.

Improvement of the AOAC 2009.01 total dietary fibre method for bread and other high starch containing matrices.

Brunt, K. & Sanders, P. (2013). Food Chemistry, 140(3), 574-580.

Responses of Senna reticulata, a legume tree from the Amazonian floodplains, to elevated atmospheric CO2 concentration and waterlogging.

Arenque, B. C., Grandis, A., Pocius, O., de Souza, A. P. & Buckeridge, M. S. Trees, 28(4), 1021-1034.

Enzyme-aided investigation of the substituent distribution in cationic potato amylopectin starch.

Richardson, S., Nilsson, G., Cohen, A., Momcilovic, D., Brinkmalm, G. & Gorton, L. (2003). Analytical Chemistry, 75(23), 6499-6508.

Extraction of β-glucan from oats for soluble dietary fiber quality analysis.

Doehlert, D. C., Simsek, S. & McMullen, M. S. (2012). Cereal Chemistry, 89(5), 230-236.

Starch synthase 4 is located in the thylakoid membrane and interacts with plastoglobule‐associated proteins in Arabidopsis.

Gámez‐Arjona, F. M., Raynaud, S., Ragel, P. & Mérida, Á. (2014). The Plant Journal, 80(2), 305-316.

Efficient Synthesis of Glucosyl-β-Cyclodextrin from Maltodextrins by Combined Action of Cyclodextrin Glucosyltransferase and Amyloglucosidase.

Xia, L., Bai, Y., Mu, W., Wang, J., Xu, X. & Jin, Z. (2017). Journal of Agricultural and Food Chemistry, 65(29), 6023-6029.

High-efficiency production of γ-cyclodextrin using β-cyclodextrin as the donor raw material by cyclodextrin opening reactions using recombinant cyclodextrin glycosyltransferase.

Qiu, C., Wang, J., Fan, H., Bai, Y., Tian, Y., Xu, X. & Jin, Z. (2017). Carbohydrate Polymers, 182, 75-80.