Arabinan Assay Kit

The Arabinan test kit is suitable for the measurement and analysis of Arabinan in fruit juice concentrates.

Product Code
Content/size
Stock
Price
Qty
K-ARAB
100 assays per kit
$322.00

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UV-method for the determination of Arabinan in plant
materials and juices

Principle:
    (endo-arabinanase + α-L-arabinofuranosidase)
(1) Arabinan + H2O →  L-arabinose

              (galactose mutarotase)
(2) α-L-Arabinose ↔ β-L-arabinose

                     (β-galactose dehydrogenase)
(3) β-L-Arabinose + NAD+ → L-arabinonic acid + NADH + H+

Kit size:                           100 assays
Method:                           Spectrophotometric at 340 nm
Reaction time:                  ~ 70 min
Detection limit:                1.3 mg/L
Application examples:
Fruit juices and other materials
Method recognition:         Novel method

Advantages

  • Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
     
  • Very cost effective
     
  • All reagents stable for > 2 years after preparation
     
  • Only enzymatic kit available
     
  • Very specific
     
  • Simple format
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

Novel and selective substrates for the assay of endo-arabinanase.

McCleary, B. V. (1989). "Gums and Stabilisers for the Food Industry, Vol 5”, (G. O. Phillips, D. J. Wedlock and P. A.Williams, Eds.), IRL Press, pp. 291-298.

Comparison of endolytic hydrolases that depolymerise 1,4-β-D-mannan, 1,5-α-L-arabinan and 1,4-β-D-galactan.

McCleary, B. V. (1991). “Enzymes in Biomass Conversion”, (M. E. Himmel and G. F. Leatham, Eds.), ACS Symposium Series, 460, Chapter 34, pp. 437-449. American Chemical Society, Washington.

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FAQs