Asparaginase (E. coli

High purity recombinant Asparaginase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.5.1.1
CAS: 9015-68-3 

asparaginase; L-asparagine amidohydrolase

Recombinant. From Escherichia coli.
In 2.5 M lithium sulphate.
Supplied at ~ 350 U/mL. 

Specific activity:
~ 15 U/mg (25oC, pH 8.0 on L-asparagine);
~ 30 U/mg (37oC, pH 8.0 on L-asparagine).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-ASNEC
400 Units at 25oC
$147.00

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DESCRIPTION

Asparaginase (Escherichia coli)

EC 3.5.1.1
CAS: 9015-68-3

Synonyms:
asparaginase; L-asparagine amidohydrolase

Form:
In 2.5 M lithium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 15 U/mg (25oC, pH 8.0 on L-asparagine); 
~ 30 U/mg (37oC, pH 8.0 on L-asparagine).

Unit definition:.
One Unit of asparaginase activity is defined as the amount of enzyme required to produce one μmole of L-aspartate from L-asparagine (7.3 mM) per minute in the presence of NADPH in Tris.HCL buffer (45 mM), pH 8.0.

Specificity:
Catalyses the reaction:
L-asparagine + H2O = L-aspartate + NH3

Applications:
Applications established for the measurement of asparagine in the food, ferementation wine beverage, medical and pharmacological industries.

Sequential capillary electrophoresis analysis using optically gated sample injection and UV/vis detection.

Liu, X., Tian, M., Camara, M. A., Guo, L. & Yang, L. (2015). Electrophoresis, 36(19), 2380-2385.