Aspartame Assay Kit

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Suitable for manual, auto-analyser and microplate formats.

Content/Size SKU Price Qty In Stock
50 assays (manual) / 500 assays (microplate)
/ 500 assays (auto-analyser)
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  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Only enzymatic kit available
  • Measures aspartame and breakdown products (L-aspartate and aspartame acid)
  • Very specific
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials

                       (pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH

                      (dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine

                      (glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate

                                 (L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+

Kit size:                            50 assays (manual) / 500 (microplate)
                                         / 500 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 5 min
Detection limit:                 0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition:      Novel method

Enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase.

Wang, Z. W., Chen, Y. & Chao, Y. P. (2006). Journal of Biotechnology, 124 (2), 403-411.