Aspartame Assay Kit

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Suitable for manual, auto-analyser and microplate formats.

Product Code
Content/size
Stock
Price
Qty
K-ASPTM
50 assays (manual) / 500 assays (microplate)
/ 500 assays (auto-analyser)
$221.00

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UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials

Principle:
                       (pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH

                      (dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine

                      (glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate

                                 (L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+

Kit size:                            50 assays (manual) / 500 (microplate)
                                          / 500 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 5 min
Detection limit:                 0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition:      Novel method

Advantages

  • Very cost effective
     
  • All reagents stable for > 12 months after preparation
     
  • Only enzymatic kit available
     
  • Measures aspartame and breakdown products (L-aspartate and aspartame acid)
     
  • Very specific
     
  • Very rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase.

Wang, Z. W., Chen, Y. & Chao, Y. P. (2006). Journal of Biotechnology, 124 (2), 403-411.

Q1. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

Q3. There is an issue with the performance of the kit; the results are not as expected.

Q4. How much sample should be used for the clarification/extraction of my sample?

Q5. Can the sensitivity of the kit assay be increased?

Q6. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

Q7. Can the manual assay format be scaled down to a 96-well microplate format?

Q8. Can the sensitivity of the kit assay be increased?

Q9. How much sample should be used for the clarification/extraction of my sample?

Q10. I have some doubts about the appearance/quality of a kit component what should be done?

Q11. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Q12. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Q13. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

Q14. Must the minimum absorbance change for a sample always be at least 0.1?

Q15. Can the sensitivity of the kit assay be increased?