Azo-Barley Glucan

High purity dyed, soluble Azo-Barley Glucan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

Highly purified, low viscosity barley 1,3:1,4-β-D-glucan dyed with Remazol Brilliant Blue R dye. Recommended substrate for the measurement of β-glucanase in malt flour.

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Product Code
100 mL (1% w/v)

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Novel approaches to the automated assay of β-glucanase and lichenase activity.

Mangan, D., Liadova, A., Ivory, R. & McCleary, B. V. (2016). Carbohydrate Research, 435, 162-172.

Measurement of malt beta-glucanase.

McCleary, B. V. (1986). Proceedings of the 19th Convention of the Institute of Brewing (Aust. and N.Z. section), 181-187.

A soluble chromogenic substrate for the assay of (1→3)(1→4)-β-D-glucanase (lichenase).

McCleary, B. V. (1986). Carbohydrate Polymers, 6(4), 307-318.

Problems caused by barley beta-glucans in the brewing industry.

McCleary, B. V. (1986). Chemistry in Australia, 53, 306-308.

Assay of malt β-glucanase using azo-barley glucan: an improved precipitant.

McCleary, B. V. & Shameer, I. (1987). Journal of the Institute of Brewing, 93(2), 87-90.

Effect of high hydrostatic pressure-temperature combinations on the activity of β-Glucanase from barley malt.

Buckow, R., Heinz, V. & Knorr, D. (2005). Journal Institute Brewing, 111(3), 282–289.

Effect of the use of dilute alkaline prior to Bacillus subtilis based biocontrol steeping and germination conditions on red sorghum malt β-glucanase activities and residual β-glucans.

Bwanganga Tawaba, J. C., Destain, J., Malumba Kamba, P., Béra, F. & Thonart, P. (2013). Journal of Cereal Science, 58(1), 148-155.

Retinol-induced secretion of human retinol-binding protein in yeast.

Reppe, S., Smeland, S., Moskaugb, J. & Blomhof, R. (1998). FEBS Letters, 427(2), 213–219.

Supplements of transgenic malt or grain containing (1, 3-1, 4)-β-glucanase increase the nutritive value of barley-based broiler diets to that of maize.

Von Wettstein, D., Warner, J. & Kannangara, C. G. (2003). British Poultry Science, 44(3), 438-449.

Assessment of enzymatic endosperm modification of malting barley using individual grain analyses.

De Sá, R. M. & Palmer, G. H. (2004). Journal of the Institute of Brewing, 110(1), 43-50.

Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi.

Shen, H., Gilkes, N. R., Kilburn, D. G., Miller Jr, R. C. & Warren, R. A. (1995). Biochemical Journal, 311, 67-74.

Production of a thermostable 1,3-1,4-β-glucanase mutant in Bacillus subtilis WB600 at a high fermentation capacity and its potential application in the brewing industry.

Niu, C., Liu, C., Li, Y., Zheng, F., Wang, J. & Li, Q. (2017). International Journal of Biological Macromolecules, 107, 28-34.

Comparison of releasing bound phenolic acids from wheat bran by fermentation of three Aspergillus species.

Yin, Z., Wu, W., Sun, C., Lei, Z., Chen, H., Liu, H., Chen, W., Ma, J., Min, T., Zhang, M. & Wu, H. (2017). International Journal of Food Science & Technology, In Press.
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Chapter 1: Principle of the Assay Procedure
Chapter 2: Substrate & Kit Description
Chapter 3: Dissolution of Azo-CM-Cellulose
Chapter 4: Precipitant Solution
Chapter 5: Preparation of Buffer Solution
Chapter 6: Assay Procedure
Chapter 7: Calculation

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