Azo-Barley Glucan

High purity dyed, soluble Azo-Barley Glucan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

Highly purified, low viscosity barley 1,3:1,4-β-D-glucan dyed with Remazol Brilliant Blue R dye. Recommended substrate for the measurement of β-glucanase in malt flour.

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Product Code
Content/size
Stock
Price
Qty
S-ABG100
100 mL (1% w/v)
$215.00

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Novel approaches to the automated assay of β-glucanase and lichenase activity.

Mangan, D., Liadova, A., Ivory, R. & McCleary, B. V. (2016). Carbohydrate Research, 435, 162-172.

Measurement of malt beta-glucanase.

McCleary, B. V. (1986). Proceedings of the 19th Convention of the Institute of Brewing (Aust. and N.Z. section), 181-187.

A soluble chromogenic substrate for the assay of (1→3)(1→4)-β-D-glucanase (lichenase).

McCleary, B. V. (1986). Carbohydrate Polymers, 6(4), 307-318.

Problems caused by barley beta-glucans in the brewing industry.

McCleary, B. V. (1986). Chemistry in Australia, 53, 306-308.

Assay of malt β-glucanase using azo-barley glucan: an improved precipitant.

McCleary, B. V. & Shameer, I. (1987). Journal of the Institute of Brewing, 93(2), 87-90.

Effect of high hydrostatic pressure-temperature combinations on the activity of β-Glucanase from barley malt.

Buckow, R., Heinz, V. & Knorr, D. (2005). Journal Institute Brewing, 111(3), 282–289.

Effect of the use of dilute alkaline prior to Bacillus subtilis based biocontrol steeping and germination conditions on red sorghum malt β-glucanase activities and residual β-glucans.

Bwanganga Tawaba, J. C., Destain, J., Malumba Kamba, P., Béra, F. & Thonart, P. (2013). Journal of Cereal Science, 58(1), 148-155.

Retinol-induced secretion of human retinol-binding protein in yeast.

Reppe, S., Smeland, S., Moskaugb, J. & Blomhof, R. (1998). FEBS Letters, 427(2), 213–219.

Supplements of transgenic malt or grain containing (1, 3-1, 4)-β-glucanase increase the nutritive value of barley-based broiler diets to that of maize.

Von Wettstein, D., Warner, J. & Kannangara, C. G. (2003). British Poultry Science, 44(3), 438-449.

Assessment of enzymatic endosperm modification of malting barley using individual grain analyses.

De Sá, R. M. & Palmer, G. H. (2004). Journal of the Institute of Brewing, 110(1), 43-50.

Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi.

Shen, H., Gilkes, N. R., Kilburn, D. G., Miller Jr, R. C. & Warren, R. A. (1995). Biochemical Journal, 311, 67-74.

Production of a thermostable 1,3-1,4-β-glucanase mutant in Bacillus subtilis WB600 at a high fermentation capacity and its potential application in the brewing industry.

Niu, C., Liu, C., Li, Y., Zheng, F., Wang, J. & Li, Q. (2017). International Journal of Biological Macromolecules, In Press.
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Chapter 1: Principle of the Assay Procedure
Chapter 2: Substrate & Kit Description
Chapter 3: Dissolution of Azo-CM-Cellulose
Chapter 4: Precipitant Solution
Chapter 5: Preparation of Buffer Solution
Chapter 6: Assay Procedure
Chapter 7: Calculation

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs