Azo-Carob Galactomannan

High purity dyed, soluble Azo-Carob Galactomannan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. 

Substrate for the specific measurement of endo-1,4-β-D-mannanase.

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Product Code
4 grams

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A simple assay procedure for β-D-mannanase.

McCleary, B. V. (1978). Carbohydrate Research, 67(1), 213-221.

Regulation of endo-acting glycosyl hydrolases in the hyperthermophilic bacterium Thermotoga maritima grown on glucan- and mannan-based polysaccharides.

Chhabra, S. R., Shockley, K. R., Ward, D. E. & Kelly, R. M.(2002). Applied and Environmental Microbiology, 68(2), 545-554.

Effect of enzyme treatment on chemical composition and production of reducing sugars in palm (Elaeis guineenis) kernel expeller.

Saenphoom, P., Liang, J. B., Ho, Y. W., Loh, T. C. & Rosfarizan, M. (2011). African Journal of Biotechnology, 10(68), 15372-15377,

Indirect method for quantification of cell biomass during solid-state fermentation of palm kernel cake based on protein content.

Abd-Aziz, S., Hung, G. S., Hassan, M. A., Karim, M. I. A. & Samat, N. (2008). Asian Journal of Scientific Research, 1(4), 385-393.

Isolation, screening and identification of mannanase producer microorganisms (Pemencilan, penyaringan dan pengenalpastian mikroorganisma penghasil enzim mannanase).

Asfamawi, K. K., Noraini, S. & Darah, I. (2013). Journal of Tropical Agriculture and Food Science, 41(1), 169– 177.

A family 26 mannanase produced by Clostridium thermocellum as a component of the cellulosome contains a domain which is conserved in mannanases from anaerobic fungi.

Halstead, J. R., Vercoe, P. E. , Gilbert, H. J. , Davidson, K. & Hazlewood, G. P. (1999). Microbiology, 145, 3101–3108.

Characterization of salt-adapted secreted lignocellulolytic enzymes from the mangrove fungus Pestalotiopsis sp.

Arfi, Y., Chevret, D., Henrissat, B., Berrin, J. G., Levasseur, A. & Record, E. (2013). Nature Communications, 4, Article Number 1810.

Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii Using palm kernel cake as carbon source.

Abd-Aziz, S., Ab-Razak, N. A., Musa, M. H. & Hassam, M. A. (2009). Research Journal of Environmental Sciences, 3(2), 251-256.

Crystallization and preliminary X-ray diffraction studies of a family 26 endo-β-1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa.

Scott, M., Pickersgill , R. W., Hazelwood, G. P., Bolam, D., Gilbert, H. J. & Harris, G. W. (1998). Acta Crystallographica, D54, 129-131.

Mannan-degrading enzymes from Cellulomonas fimi.

Stoll, D. Stalbrand, H., Warren, R. A. J. (1999). Journal of Applied and Environmental Microbiology, 65(6), 2598-2605.

Evaluation of Macerating Pectinase Enzyme Activity under Various Temperature, pH and Ethanol Regimes.

Reynolds, A. G., Knox, A. & Di Profio, F. (2018). Beverages, 4(1), 10.
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Principle of the Assay Procedure
Chapter 2: Substrate & Kit Description
Chapter 3: Dissolution of Azo-CM-Cellulose
Chapter 4: Precipitant Solution
Chapter 5: Preparation of Buffer Solution
Chapter 6: Assay Procedure
Chapter 7: Calculation

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