Azo-Casein (Sulphanilamide Dyed)

High purity dyed, soluble Azo-Casein (Sulphanilamide Dyed) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

A highly sensitive, soluble substrate for the assay of endo-protease activity. This substrate has a 5-fold greater sensitivity than similar products supplied by other companies.

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Product Code
10 grams

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Harvested broccoli (Brassica oleracea) responds to high carbon dioxide and low oxygen atmosphere by inducing stress-response genes.

Eason, J. R., Ryan, D., Page, B., Watson, L. & Coupe, S. A. (2007). Postharvest Biology and Technology, 43(3), 358-365.

Programmed cell death during flower senescence: isolation and characterization of cysteine proteinases from Sandersonia aurantiaca.

Eason, J. R., Ryan, D. J., Pinkney, T. T. & O'Donoghue, E. M. (2002). Functional Plant Biology, 29(9), 1055-1064.

Effect of pH on the solubilization of brewers’ spent grain by microbial carbohydrases and proteases.

Faulds, C. B., Robertson, J. A. & Waldron, K. W. (2008). Journal of Agricultural and Food Chemistry, 56(16), 7038-7043.

Protease-induced solubilisation of carbohydrates from brewers' spent grain.

Faulds, C. B., Collins, S., Robertson, J. A., Treimo, J., Eijsink, V. G. H., Hinz, S. W. A. Schols, H. A., Buchert, J. & Waldron, K. W. (2009). Journal of Cereal Science, 50(3), 332-336.

Isolation and characterization of a Bacillus licheniformis strain capable of degrading zearalenone.

Yi, P. J., Pai, C. K. & Liu, J. R. (2011). World Journal of Microbiology and Biotechnology, 27(5), 1035-1043.

Suppression of the cysteine protease, aleurain, delays floret senescence in Brassica oleracea.

Eason, J. R., Ryan, D. J., Watson, L. M., Hedderley, D., Christey, M. C., Braun, R. H. & Coupe, S. A. (2005). Plant Molecular Biology, 57(5), 645-657.

Hydrolysis of raw hide using proteolytic enzyme extracted from papaya latex.

Pitpreecha, S. & Damrongsakkul, S. (2006). Korean Journal of Chemical Engineering, 23(6), 972-976.

Identification of dehydration‐responsive cysteine proteases during post‐harvest senescence of broccoli florets.

Coupe, S. A., Sinclair, B. K., Watson, L. M., Heyes, J. A. & Eason, J. R. (2003). Journal of Experimental Botany, 54(384), 1045-1056.

Suppressing expression of a soluble acid invertase (BoINV2) in broccoli (Brassica oleracea) delays postharvest floret senescence and downregulates cysteine protease (BoCP5) transcription.

Eason, J. R., Ryan, D. J., Watson, L. M., Pinkney, T., Hedderley, D., Christey, M. C., Braun, R. H. & Coupe, S. A. (2007). Physiologia Plantarum, 130(1), 46-57.

Effects of soluble dietary cellulose on specific growth rate, survival and digestive enzyme activities in three freshwater crayfish (Cherax) species.

Dammannagoda, L. K., Pavasovic, A., Hurwood, D. A. & Mather, P. B. (2015). Aquaculture Research, 46(3), 626-636.

Characterisation of the enzymatic properties of MpAPr1, an aspartic protease secreted by the wine yeast Metschnikowia pulcherrima.

Theron, L. W., Bely, M. & Divol, B. (2017). Journal of the Science of Food and Agriculture.

Batch-to-batch variation and storage stability of the commercial peptidase preparation Flavourzyme in respect of key enzyme activities and its influence on process reproducibility.

Merz, M., Appel, D., Berends, P., Rabe, S., Blank, I., Stressler, T. & Fischer, L. (2016). European Food Research and Technology, 242(7), 1005-1012.
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Principle of the Assay Procedure
Chapter 2: Substrate & Kit Description
Chapter 3: Dissolution of Azo-CM-Cellulose
Chapter 4: Precipitant Solution
Chapter 5: Preparation of Buffer Solution
Chapter 6: Assay Procedure
Chapter 7: Calculation

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.