Azo-Wheat Arabinoxylan (Liquid)

High purity dyed, soluble Azo-Wheat Arabinoxylan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared by dyeing wheat flour arabinoxylan with Remazol Brilliant Blue R dye. Substrate for the assay of endo-1,4-β-D-xylanase.

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Product Code
100 mL (1% w/v)

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Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Evidence for temporal regulation of the two Pseudomonas cellulosa xylanases belonging to glycoside hydrolase family 11.

Emami, K., Nagy, T., Fontes, C. M. G. A., Ferreira, L. M.A. & Gilbert, H. J. (2002). Journal of Bacteriology, 184(15), 4124-4133.

Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases.

Cuyvers, S., Dornez, E., Rezaei, M. N., Pollet, A., Delcour, J. A. & Courtin, C. M. (2011). FEBS Journal, 278(7), 1098–1111.

Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem.

Grootaert, C., Van den Abbeele, P., Marzorati, M., Broekaert, W. F., Courtin, C. M., Delcour, J. A., Verstraete, W. & Van de Wiele, T. (2009). FEMS Microbiology Ecology, 69(2), 231–242.

Arabinoxylan‐oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the simulator of human intestinal microbial ecosystem.

Sanchez, J. I., Marzorati, M., Grootaert, C., Baran, M., Van Craeyveld, V., Courtin, C. M., Broekaet, W. F. Declour, J. A.,Verstraete, W. & Van de Wiele, T. (2009). Microbial Biotechnology, 2(1), 101-113.

Microbial community development in a dynamic gut model is reproducible, colon region specific, and selective for bacteroidetes and clostridium cluster IX.

Van den Abbeele, P., Grootaert, C., Marzorati, M., Possemiers, S., Verstraete, W., Gérard, P., Rabot, S., Bruneau, A., El Aidy, S., Derrien, M., Zoetendal, E., Kleerebezem, M., Smidt, H. & Van de Wiele, T. (2010). Applied and Environmental Microbiology, 76(15), 5237-5246.

Glycoside Hydrolases from a targeted Compost Metagenome, activity-screening and functional characterization.

Dougherty, M. J., D’haeseleer P., Hazen, T. C., Simmons, B. A., Adams, P. D. & Hadi, M. Z. (2012). BMC Biotechnology, 12, 38.

Hemicellulase production in Chrysosporium lucknowense C1.

Hinz, S. W. A., Pouvreau, L., Joosten, R., Bartels, J., Jonathan, M. C., Wery, J. & Schols, H. A. (2009). Journal of Cereal Science, 50(3), 318-323.

Impact of cell wall‐degrading enzymes on water‐holding capacity and solubility of dietary fibre in rye and wheat bran.

Petersson, K., Nordlund, E., Tornberg, E., Eliasson, A. C., & Buchert, J. (2013). Journal of the Science of Food and Agriculture, 93(4), 882-889.

Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris.

Chang, X., Xu, B., Bai, Y., Luo, H., Ma, R., Shi, P. & Yao, B. (2017). PloS One, 12(2), e0171111.

Management of enzyme diversity in high-performance cellulolytic cocktails.

Reyes-Sosa, F. M., Morales, M. L., Gómez, A. I. P., Crespo, N. V., Zamorano, L. S., Rocha-Martín, J., Molina-Heredia, F. P. & García, B. D. (2017). Biotechnology for Biofuels, 10(1), 156.
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Principle of the Assay Procedure
Chapter 2: Substrate & Kit Description
Chapter 3: Dissolution of Azo-CM-Cellulose
Chapter 4: Precipitant Solution
Chapter 5: Preparation of Buffer Solution
Chapter 6: Assay Procedure
Chapter 7: Calculation

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