β-Glucosidase (Aspergillus niger

High purity β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.21
CAZy Family: GH3
CAS: 9001-22-3

beta-glucosidase; beta-D-glucoside glucohydrolase

Highly purified. From Aspergillus niger.
In 3.2 M ammonium sulphate (stabilised with BSA).
Supplied at ~ 40 U/mL. 

Specific activity: ~ 50 U/mg (40oC, pH 4.0 on p-nitrophenyl β-glucoside).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-BGLUC
200 Units
$290.00

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DESCRIPTION

β-Glucosidase (Aspergillus niger)

EC 3.2.1.21
CAZy Family: GH3
CAS: 9001-22-3

Synonyms:
beta-glucosidase; beta-D-glucoside glucohydrolase

Form:
In 3.2 M ammonium sulphate (stabilised with BSA).

Stability: 
> 4 years at 4oC.

Specific activity:
~ 50 U/mg (40°C, pH 4.0 on p-nitrophenyl β-glucoside).

Unit definition:
One Unit of β-Glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol from p-nitrophenyl β-glucoside per minute at 40oC and pH 4.0.

Specificity:
Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose.

Applications:
Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.

Purification of β-D-glucosidase from Aspergillus niger.

McCleary, B. V. & Harrington, J. (1988). “Methods in Enzymology”, Volume 160, (H. Gilbert, Ed.), Elsevier Inc., pp. 575-583.

Enzymic quantification of (1→3) (1→4)-β-D-glucan in barley and malt.

McCleary, B. V. & Glennie-Holmes, M. (1985). Journal of the Institute of Brewing, 91(5), 285-295.

Measurement of (1→3)(1→4)-β-D-glucan in malt, wort and beer.

McCleary, B. V. & Nurthen, E. (1986). Journal of the Institute of Brewing, 92(2), 168-173.

Enzymic hydrolysis and industrial importance of barley β-glucans and wheat flour pentosans.

McCleary, B. V., Gibson, T. S., Allen, H. & Gams, T. C. (1986). Starch-Stärke, 38(12), 433-437.

Measurement of (1→3),(1→4)-β-D-glucan in barley and oats: A streamlined enzymic procedure.

McCleary, B. V. & Codd, R. (1991). Journal of the Science of Food and Agriculture, 55(2), 303-312.

Enzymatic preparation of mushroom dietary fibre: A comparison between analytical and industrial enzymes.

Wong, K. H. & Cheung, P. C. K. (2009). Food Chemistry, 115(3), 795-800.

Enzymatic hydrolysis of steryl ferulates and steryl glycosides.

Nyström, L., Moreau, R. A., Lampi, A. M., Hicks, K. B. & Piironen, V. (2008). European Food Research and Technology, 227(3), 727-733.

Formulation of enzyme blends to maximize the hydrolysis of alkaline peroxide pretreated alfalfa hay and barley straw by rumen enzymes and commercial cellulases.

Badhan, A., Wang, Y., Gruninger, R., Patton, D., Powlowski, J., Tsang, A. & McAllister, T. (2014). BMC Biotechnology, 14(1), 31.

Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans.

Rieder, A., Grimmer, S., Aachmann, F. L., Westereng, B., Kolset, S. O. & Knutsen, S. H. (2013). Carbohydrate Polymers, 92(2), 2075-2083.

Biomass hydrolyzing enzymes from plant pathogen Xanthomonas axonopodis pv. punicae: optimizing production and characterization.

Amat, D., Arora, A., Nain, L. & Saxena, A. K. (2014). Annals of Microbiology, 64(1), 267-274.

Droplet-based microfluidic platform for heterogeneous enzymatic assays.

Chang, C., Sustarich, J., Bharadwaj, R., Chandrasekaran, A., Adams, P. D. & Singh, A. K. (2013). Lab Chip, 13(9), 1817-1822.

High-throughput enzymatic hydrolysis of lignocellulosic biomass via in-situ regeneration.

Bharadwaj, R., Wong, A., Knierim, B., Singh, S., Holmes, B. M., Auer, M., Simmons, B. A., Adams, P. D. & Singh, A. K. (2011). Bioresource Technology, 102(2), 1329-1337.

Pretreatment of paddy straw with Trametes hirsuta for improved enzymatic saccharification.

Saritha, M., Arora, A. & Nain, L. (2012). Bioresource Technology, 104, 459-465.

Substrate specificities of glycosidases from Aspergillus species pectinase preparations on elderberry anthocyanins.

Pricelius, S., Murkovic, M., Souter, P. & Guebitz, G. M. (2009). Journal of Agricultural and Food Chemistry, 57(3), 1006-1012.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.