β-Glucosidase (Thermostable) (Thermotoga maritima

High purity recombinant β-Glucosidase (thermostable) (Thermotoga  maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.21
CAZy Family: GH1
CAS: 9001-22-3

beta-glucosidase; beta-D-glucoside glucohydrolase

Recombinant. From Thermotoga maritima.
In 3.2 M ammonium sulphate.
Supplied at ~ 460 U/mL. 

Specific activity:
~ 70 U/mg (40oC, pH 6.5 on p-nitrophenyl β-D-glucopyranoside).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-BGOSTM
460 Units
$260.00

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DESCRIPTION

β-Glucosidase (thermostable) (Thermotoga maritima)

EC 3.2.1.21
CAZy Family: GH1
CAS: 9001-22-3

Synonyms:
beta-glucosidase; beta-D-glucoside glucohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 70 U/mg (40oC, pH 6.5 on p-nitrophenyl β-D-glucopyranoside).

Unit definition:.
One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-glucopyranoside (10 mM) in sodium maleate buffer (50 mM), pH 6.5 at 40oC.

Specificity:
Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose.

Applications:
Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.

Colourimetric and fluorimetric substrates for the assay of limit dextrinase.

Mangan, D., McCleary, B. V., Cornaggia, C., Ivory, R., Rooney, E. & McKie, V. (2015). Journal of Cereal Science, 62, 50-57.