β-Mannosidase (Cellulomonas fimi

High purity recombinant β-Mannosidase (Cellilomonas fimi) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.25
CAZy Family: GH2
CAS: 9025-43-8

beta-mannosidase; beta-D-mannoside mannohydrolase

Recombinant. From Cellulomonas fimi.
In 3.2 M ammonium sulphate.
Supplied at ~ 80 U/mL. 

Specific activity:
~ 15 U/mg (35oC, pH 6.5 on p-nitrophenyl β-D-mannopyranoside).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-BMOSCF
200 Units
$226.00

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DESCRIPTION

β-Mannosidase (Cellulomonas fimi)

EC 3.2.1.25 
CAZy Family: GH2
CAS: 9025-43-8

Synonyms:
beta-mannosidase; beta-D-mannoside mannohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 15 U/mg (35oC, pH 6.5 on p-nitrophenyl β-D-mannopyranoside).

Unit definition:.
One Unit of β-D-mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-mannopyranoside (0.8 mM) in sodium maleate buffer (100 mM), pH 6.5 at 35oC.

Specificity:
Hydrolysis of terminal, non-reducing β-D-mannose residues in β-D-mannosides.

Applications:
Applications in carbohydrate and biofuels research.

Galactomannan structure and β-mannanase and β-mannosidase activity in germinating legume seeds.

McCleary, B. V. & Matheson, N. K. (1975). Phytochemistry, 14(5-6), 1187-1194.

Galactomannans and a galactoglucomannan in legume seed endosperms: Structural requirements for β-mannanase hydrolysis.

McCleary, B. V., Matheson, N. K. & Small, D. B. (1976). Phytochemistry, 15(7), 1111-1117.

Modes of action of β-mannanase enzymes of diverse origin on legume seed galactomannans.

McCleary, B. V. (1979). Phytochemistry, 18(5), 757-763.

An enzymic technique for the quantitation of galactomannan in guar Seeds.

McCleary, B. V. (1981). Lebensmittel-Wissenschaft & Technologie, 14, 56-59.

Purification and properties of a β-D-mannoside mannohydrolase from guar.

McCleary, B. V. (1982), Carbohydrate Research, 101(1), 75-92.

Preparative–scale isolation and characterisation of 61-α-D-galactosyl-(1→4)-β-D-mannobiose and 62-α-D-galactosyl-(1→4)-β-D-mannobiose.

McCleary, B. V., Taravel, F. R. & Cheetham, N. W. H. (1982). Carbohydrate Research, 104(2), 285-297.

Characterisation of the oligosaccharides produced on hydrolysis of galactomannan with β-D-mannase.

McCleary, B. V., Nurthen, E., Taravel, F. R. & Joseleau, J. P. (1983). Carbohydrate Research, 118, 91-109.

Action patterns and substrate-binding requirements of β-D-mannanase with mannosaccharides and mannan-type polysaccharides.

McCleary, B. V. & Matheson, N. K. (1983). Carbohydrate Research, 119, 191-219.

The fine structures of carob and guar galactomannans.

McCleary, B. V., Clark, A. H., Dea, I. C. M. & Rees, D. A. (1985). Carbohydrate Research, 139, 237-260.

Effect of galactose-substitution-patterns on the interaction properties of galactomannas.

Dea, I. C. M., Clark, A. H. & McCleary, B. V. (1986). Carbohydrate Research, 147(2), 275-294.

Effect of the molecular fine structure of galactomannans on their interaction properties - the role of unsubstituted sides.

Dea, I. C. M., Clark, A. H. & McCleary, B. V. (1986). Food Hydrocolloids, 1(2), 129-140.

Galactomannan changes in developing Gleditsia Triacanthos Seeds.

McCleary, B. V., Mallett, I. & Matheson, N. K. (1987). Phytochemistry, 26(7), 1889-1894.