β-Xylosidase (Bacillus pumilus

High purity recombinant β-Xylosidase (Bacillus pumilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.37
CAZy Family: GH43
CAS: 9025-53-0

xylan 1,4-beta-xylosidase; 4-beta-D-xylan xylohydrolase

Recombinant. From Bacillus pumilus.
In 3.2 M ammonium sulphate.
Supplied at ~ 75 U/mL. 

Specific activity:
~ 18 U/mg (35oC, pH 7.5 on p-nitrophenyl-β-D-xylopyranoside).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-BXSEBP
200 Units
$201.00

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DESCRIPTION

β-Xylosidase (Bacillus pumilus)

EC 3.2.1.37 
CAZy Family: GH43
CAS: 9025-53-0

Synonyms:
xylan 1,4-beta-xylosidase; 4-beta-D-xylan xylohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 18 U/mg (35oC, pH 7.5 on p-nitrophenyl-β-D-xylopyranoside).

Unit definition:
One Unit of β-xylosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-Dxylopyranoside (5 mM) in potassium phosphate buffer (50 mM), pH 7.5 at 35oC.

Specificity:
Hydrolysis of (1,4)-β-D-xylans and xylo-oligosaccharides to remove successive D-xylose residues from non-reducing termini. 

Applications:
Applications in carbohydrate and biofuels research.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.

Highly active β-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates.

Jordan, D. B., Wagschal, K., Grigorescu, A. A. & Braker, J. D. (2013). Applied Microbiology and Biotechnology, 97(10), 4415-4428.

Trans-α-xylosidase, a widespread enzyme activity in plants, introduces (1→ 4)-α-D-xylobiose side-chains into xyloglucan structures.

Franková, L. & Fry, S. C. (2012). Phytochemistry, 78, 29-43.

Trans‐α‐xylosidase and trans‐β-galactosidase activities, widespread in plants, modify and stabilize xyloglucan structures.

Franková, L. & Fry, S. C. (2012). The Plant Journal, 71(1), 45-60.