Cellafluor Reagent

Cellafluor is a fluorimetric reagent that can be used for the assay of cellulase (endo-1,4-β-glucanase). The reagent consists of 2 components:

1)      4,6-O-Benzylidene-4-methylumbelliferyl-β-cellotrioside

2)      β-glucosidase (Thermotoga maritima)

Upon incubation with a cellulase (endo-1,4-β-glucanase) enzyme, the functionalised trisaccharide is cleaved and the ancillary β-glucosidase can act to hydrolyse the fragment containing the 4-methylumbelliferyl group. The rate of formation of 4-methylumbelliferyl is directly proportional to the cellulase (endo-1,4-β-glucanase) activity. The assay reaction is terminated and full fluorescence obtained from the fluorophore by the addition of Tris buffer.

Product Code
Content/size
Stock
Price
Qty
R-CELLFLR
100 assays (manual) / 1000 assays (auto-analyser) / 1000 assays (microplate)
$196.00

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Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase.

Mangan, D., McCleary, B. V., Liadova, A., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 395, 47-51.

Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase).

McCleary, B. V., Mangan, D., Daly, R., Fort, S., Ivory, R. & McCormack, N. (2014), Carbohydrate Research, 385, 9-17.