Cellulase (endo-1,4-β-D-glucanase) (Aspergillus niger

High purity Cellulase (endo-1,4-β-D-glucanase) (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4
CAZy Family: GH12
CAS: 9012-54-8

cellulase; 4-beta-D-glucan 4-glucanohydrolase

Highly purified. From Aspergillus niger.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,200 U/mL. 

Specific activity:
~ 80 U/mg (40oC, pH 4.5 on CM-cellulose 4M).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-CELAN
2,000 Units
$192.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

Cellulase (endo-1,4-β-D-glucanase) (Aspergillus niger)

EC 3.2.1.4
CAZy Family: GH12
CAS: 9012-54-8

Synonyms:
cellulase; 4-beta-D-glucan 4-glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 80 U/mg (40oC, pH 4.5 on CM-cellulose 4M).

Unit definition:
One Unit of cellulase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from CM-cellulose 4M (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.

Specificity:
endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.

Applications:
Applications established in diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries.

Measurement of endo-1,4-β-glucanase.

McCleary, B. V., McKie, V. & Draga, A. (2012). “Methods in Enzymology”, Volume 510, (H. Gilbert, Ed.), Elsevier Inc., pp. 1-17.

Measurement of polysaccharide-degrading enzymes in plants using chromogenic and colorimetric substrates.

McCleary, B. V. (1995). “New Diagnostics in Crop Sciences”, (J. R. Skerritt and R. Appels, Eds.), CAB International, pp. 277-301.

New developments in the measurement of α-amylase, endo-protease, β-glucanase and β-xylanase.

McCleary, B. V. & Monaghan, D. (2000). “Proceedings of the Second European Symposium on Enzymes in Grain Processing”, (M. Tenkanen, Ed.), VTT Information Service, pp. 31-38.

Measurement of polysaccharide degrading enzymes using chromogenic and colorimetric substrates.

McCleary, B. V. (1991). Chemistry in Australia, September, 398-401.

Pretreatment of paddy straw with Trametes hirsuta for improved enzymatic saccharification.

Saritha, M., Arora, A. & Nain, L. (2012). Bioresource Technology, 104, 459-465.

Biomass hydrolyzing enzymes from plant pathogen Xanthomonas axonopodis pv. punicae: optimizing production and characterization.

Amat, D., Arora, A., Nain, L. & Saxena, A. K. (2014). Annals of Microbiology, 64(1), 267-274.

Experimental study on the performance of microwave assisted Hydrogen peroxide (H2O2) pretreatment of rice straw.

Singh, R., Tiwari, S., Srivastava, M. & Shukla, A. (2014). Agricultural Engineering International: CIGR Journal, 16(1), 173-181.

A revised architecture of primary cell walls based on biomechanical changes induced by substrate-specific endoglucanases.

Park, Y. B. & Cosgrove, D. J. (2012). Plant Physiology, 158(4), 1933-1943.

Droplet-based microfluidic platform for heterogeneous enzymatic assays.

Chang, C., Sustarich, J., Bharadwaj, R., Chandrasekaran, A., Adams, P. D. & Singh, A. K. (2013). Lab Chip, 13(9), 1817-1822.

High-throughput enzymatic hydrolysis of lignocellulosic biomass via in-situ regeneration.

Bharadwaj, R., Wong, A., Knierim, B., Singh, S., Holmes, B. M., Auer, M., Simmons, B. A., Adams, P. D. & Singh, A. K. (2011). Bioresource Technology, 102(2), 1329-1337.

Polysaccharides from Enteromorpha compressa: Isolation, purification and structural features.

Ray, B. (2006). Carbohydrate Polymers, 66(3), 408-416.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.

Xyloglucans from flaxseed kernel cell wall: Structural and conformational characterisation.

Ding, H. H., Cui, S. W., Goff, H. D., Chen, J., Guo, Q. & Wang, Q. (2016). Carbohydrate Polymers, 151, 538-545.

Bonds broken and formed during the mixed-linkage glucan: xyloglucan endotransglucosylase reaction catalysed by Equisetum hetero-trans-β-glucanase.

Simmons, T. J. & Fry, S. C. (2017). Biochemical Journal, 474(7), 1055-1070.

Microwave Assisted Chemical Pretreatment Method for Bio-ethanol Production from Rice Straw.

Singh, R., Srivastava, M., Rohatgi, B., Kar, A. & Shukla, A. (2017). Asian Journal of Chemistry, 29(5), 943.