Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens

High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4
CAZy Family: GH5
CAS: 9012-54-8

cellulase; 4-beta-D-glucan 4-glucanohydrolase

Recombinant. From Bacillus amyloliquefaciens.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,400 U/mL. 

Specific activity:
~ 80 U/mg (40oC, pH 6.0 on CM-cellulose 4M);
~ 160 U/mg (60oC, pH 6.0 on CM-cellulose 4M).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-CELBA
3,500 Units at 40oC;
7,100 Units at 60oC
$216.00

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DESCRIPTION

Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens)

EC 3.2.1.4
CAZy Family: GH5
CAS: 9012-54-8

Synonyms:
cellulase; 4-beta-D-glucan 4-glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 80 U/mg (40oC, pH 6.0 on CM-cellulose 4M);
~ 160 U/mg (60oC, pH 6.0 on CM-cellulose 4M).

Unit definition:
One Unit of cellulase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from CM-Cellulose 4M (10 mg/mL) in sodium phosphate buffer (100 mM), pH 6.

Specificity:
endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.

Applications:
Applications established in diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries.

Measurement of endo-1,4-β-glucanase.

McCleary, B. V., McKie, V. & Draga, A. (2012). “Methods in Enzymology”, Volume 510, (H. Gilbert, Ed.), Elsevier Inc., pp. 1-17.

Measurement of polysaccharide-degrading enzymes in plants using chromogenic and colorimetric substrates.

McCleary, B. V. (1995). “New Diagnostics in Crop Sciences”, (J. R. Skerritt and R. Appels, Eds.), CAB International, pp. 277-301.

New developments in the measurement of α-amylase, endo-protease, β-glucanase and β-xylanase.

McCleary, B. V. & Monaghan, D. (2000). “Proceedings of the Second European Symposium on Enzymes in Grain Processing”, (M. Tenkanen, Ed.), VTT Information Service, pp. 31-38.

Measurement of polysaccharide degrading enzymes using chromogenic and colorimetric substrates.

McCleary, B. V. (1991). Chemistry in Australia, September, 398-401.

A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells.

Park, S., Szumlanski, A. L., Gu, F., Guo, F. & Nielsen, E. (2011). Nature Cell Biology, 13(8), 973-980.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.

A comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes.

Pierce, B. C., Agger, J. W., Zhang, Z., Wichmann, J. & Meyer, A. S. (2017). Carbohydrate Research, 449, 85-94.