Cellulase (endo-1,4-β-D-glucanase) (Talaromyces emersonii

High purity Cellulase (endo-1,4-β-D-glucanase) (Talaromyces emersonii) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4
CAZy Family: GH5
CAS: 9012-54-8

cellulase; 4-beta-D-glucan 4-glucanohydrolase

Highly purified. From Talaromyces emersonii. 
In 3.2 M ammonium sulphate.
Supplied at ~ 713 U/mL. 

Specific activity:
~ 65 U/mg (40oC, pH 4.5 on CM-cellulose 4M).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-CELTE
2,000 Units
$210.00

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DESCRIPTION

Cellulase (endo-1,4-β-D-glucanase) (Talaromyces emersonii)

EC 3.2.1.4
CAZy Family: GH5
CAS: 9012-54-8

Synonyms:
cellulase; 4-beta-D-glucan 4-glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 65 U/mg (40oC, pH 4.5 on CM-cellulose 4M).

Unit definition:
One Unit of cellulase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from CM-Cellulose 4M (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.

Specificity:
endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.

Applications:
Applications established in diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries.

Measurement of endo-1,4-β-glucanase.

McCleary, B. V., McKie, V. & Draga, A. (2012). “Methods in Enzymology”, Volume 510, (H. Gilbert, Ed.), Elsevier Inc., pp. 1-17.

Measurement of polysaccharide-degrading enzymes in plants using chromogenic and colorimetric substrates.

McCleary, B. V. (1995). “New Diagnostics in Crop Sciences”, (J. R. Skerritt and R. Appels, Eds.), CAB International, pp. 277-301.

New developments in the measurement of α-amylase, endo-protease, β-glucanase and β-xylanase.

McCleary, B. V. & Monaghan, D. (2000). “Proceedings of the Second European Symposium on Enzymes in Grain Processing”, (M. Tenkanen, Ed.), VTT Information Service, pp. 31-38.

Measurement of polysaccharide degrading enzymes using chromogenic and colorimetric substrates.

McCleary, B. V. (1991). Chemistry in Australia, September, 398-401.

A mechanistic model for rational design of optimal cellulase mixtures.

Levine, S. E., Fox, J. M., Clark, D. S. & Blanch, H. W. (2011). Biotechnology and Bioengineering, 108(11), 2561-2570.

Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production.

Chang, J. J., Ho, F. J., Ho, C. Y., Wu, Y. C., Hou, Y. H., Huang, C. C., Shih, M. C. & Li, W. H. (2013). Biotechnol Biofuels, 6(1), 19-31.