Citrate synthase (E. coli

High purity recombinant Citrate synthase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.3.3.1 (Modified from 4.1.3.7 in 2002)
CAS: 9027-96-7 

citrate (Si)-synthase; acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-S)-carboxymethyl forming]

Recombinant. From Escherichia coli.
In 3.2 M ammonium sulphate.
Supplied at ~ 300 U/mL. 

Specific activity:
~ 10 U/mg (25oC, pH 8.0 on oxaloacetic acid).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-CITEC
2,500 Units
$209.00

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DESCRIPTION

Citrate synthase (Escherichia coli)

EC 2.3.3.1 (Modified from 4.1.3.7 in 2002)
CAS: 9027-96-7

Synonyms:
citrate (Si)-synthase; acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-S)-carboxymethyl forming]

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 10 U/mg (25oC, pH 8.0 on oxaloacetic acid).

Unit definition:
One Unit of citrate synthase is defined as the amount of enzyme required to produce one μmole of citric acid from oxaloacetic acid (0.16 mM) and acetyl-CoA (0.2 mM), measured at 232nm in Tris.HCl buffer (95 mM), pH 8.0 at 25oC.

Specificity:
Catalyses the reaction:
Acetyl-CoA + H2O + oxaloacetate = citrate + CoA

Applications:
Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

Compelling advantages of negative ion mode detection in high-mass MALDI-MS for homomeric protein complexes.

Mädler, S., Barylyuk, K., Erba, E. B., Nieckarz, R. J. & Zenobi, R. (2012). Journal of The American Society for Mass Spectrometry, 23(2), 213-224.