Citrate synthase (E. coli

High purity recombinant Citrate synthase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC (Modified from in 2002)
CAS: 9027-96-7 

citrate (Si)-synthase; acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-S)-carboxymethyl forming]

Recombinant. From Escherichia coli.
In 50% (v/v) glycerol.
Supplied at ~ 300 U/mL. 

Specific activity:
~ 10 U/mg (25oC, pH 8.0 on oxaloacetic acid).

StabilityMinimum 1 year at < -10oC. Check vial for details.

Product Code
2,500 Units

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Citrate synthase (Escherichia coli)

EC (Modified from in 2002)
CAS: 9027-96-7

citrate (Si)-synthase; acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-S)-carboxymethyl forming]

In 50% (v/v) glycerol.

Minimum 1 year at < -10oC. Check vial for details.

Specific activity:
~ 10 U/mg (25oC, pH 8.0 on oxaloacetic acid).

Unit definition:
One Unit of citrate synthase is defined as the amount of enzyme required to produce one μmole of citric acid from oxaloacetic acid (0.16 mM) and acetyl-CoA (0.2 mM), measured at 232nm in Tris.HCl buffer (95 mM), pH 8.0 at 25oC.

Catalyses the reaction:
Acetyl-CoA + H2O + oxaloacetate = citrate + CoA

Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

Compelling advantages of negative ion mode detection in high-mass MALDI-MS for homomeric protein complexes.

Mädler, S., Barylyuk, K., Erba, E. B., Nieckarz, R. J. & Zenobi, R. (2012). Journal of The American Society for Mass Spectrometry, 23(2), 213-224.