D-3-Hydroxybutyric Acid Assay Kit

The D-3-Hydroxybutyric Acid Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages.

Suitable for manual, auto-analyser and microplate formats.

Product Code
60 assays (manual) / 600 assays (microplate)
/ 740 assays (auto-analyser)

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Colourimetric method for the determination of D-3-Hydroxybutyric Acid in foodstuffs

                      (3-hydroxybutyrate dehydrogenase)
(1) D-3-Hydroxybutyrate + NAD+ ↔ acetoacetate + NADH + H+

(2) INT + NADH + H+ → NAD+ + INT-formazan

Kit size:    60 assays (manual) / 600 (microplate)
                                          / 740 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 492 nm
Reaction time:                  ~ 6 min
Detection limit:                 0.074 mg/L
Application examples:
Egg, egg products (e.g. egg powder) and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by CEC


  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • No wasted diaphorase solution (stable suspension supplied)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Physiological assessment of the effects of changing water levels associated with reservoir management on fattening rates of neotropical migrants at a stopover site.

Wagner, D. N., Green, D. J., Pavlik, M., Cooper, J. & Williams, T. D. (2014). Conservation Physiology, 2(1), cou017.

The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism.

Thiel, K., Vuorio, E., Aro, E. M. & Kallio, P. T. (2017). Microbial Cell Factories, 16(1), 21.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.