D-Fructose/D-Glucose Assay Kit

D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

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Product Code
Content/size
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Price
Qty
K-FRUGL
110 assays (manual) / 1100 assays (microplate)
/ 1100 assays (auto-analyser)
$237.00

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UV-method for the determination of D-Fructose and D-Glucose
in foodstuffs, beverages and other materials

Principle:
                        (hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

                         (hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

          (glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

    (phosphoglucose isomerase)
(4) F-6-P             ↔             G-6-P

Kit size:    110 assays (manual) / 1100 (microplate)
                                          / 1100 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 13 min
Detection limit:                 0.66 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods,
bread, bakery products, candies, desserts, confectionery, ice-cream,
fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals,
paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

Advantages

  • PVP incorporated to prevent tannin inhibition
     
  • Validated by the University of Wine, Suze la Rousse, France
     
  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years after preparation (manual analysis applications)
     
  • Rapid reaction at either 25 or 37°C
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Extended cofactors stability
     
  • Suitable for manual, microplate and auto-analyser formats

Grape and wine analysis: Oenologists to exploit advanced test kits.

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Sourdough-leavened bread improves postprandial glucose and insulin plasma levels in subjects with impaired glucose tolerance.

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Metabolic and endocrine profiles in response to systemic infusion of fructose and glucose in rhesus macaques.

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Comparative effects of fructose and glucose on lipogenic gene expression and intermediary metabolism in HepG2 liver cells.

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Measurement of glucose and fructose in clinical samples using gas chromatography/mass spectrometry.

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Physiological studies of Leuconostoc mesenteroides strain NRRL B-1149 during cultivation on glucose and fructose media.

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Aldose reductase is implicated in high glucose‐induced oxidative stress in mouse embryonic neural stem cells.

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Aroma compounds in Ontario Vidal and Riesling icewines. II. Effects of crop level.

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An approach for estimating the maximum specific growth rate of Gluconobacter japonicus in strawberry purée without cell concentration data.

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Oligosaccharide Synthesis in Fruit Juice Concentrates Using a Glucansucrase From Lactobacillus reuteri 180.

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Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs