The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format)

The D-Fructose and D-Glucose MegaQuant™ Format test kit is suitable for the measurement and analysis of D-fructose and D-glucose in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm).

Product Code
60 assays per kit

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

Simple colourimetric method for the determination of
D-Fructose and D-Glucose in foodstuffs, beverages and
other materials

(1) D-Glucose + ATP → G-6-P + ADP

(2) D-Fructose + ATP → F-6-P + ADP

    (phosphoglucose isomerase)
(3) F-6-P            ↔            G-6-P

      (glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(5) INT + NADPH + H+ → NADP+ + INT-formazan

Kit size:                           60 assays
Method:                           Spectrophotometric at 505 nm
Reaction time:                 ~ 5 min
Detection limit:               23.2 mg/L
Application examples:
Grape juice / must, wine, beer, fruit juices, soft drinks, milk, jam, honey,
dietetic foods, bread, bakery products, candies, desserts, confectionery,
ice-cream, fruit and vegetables, condiments, tobacco, cosmetics,
pharmaceuticals, paper and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:      Novel method


  • Novel product, patented technology
  • Spectrophotometer / laboratory expertise not required
  • Highly stable reagents (at least three seasons use)
  • Very competitive price (cost per test)
  • Very simple procedure
  • Rapid reaction time (~ 10 min)
  • Standard included

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Production of Bioethanol from Agricultural Wastes Using Residual Thermal Energy of a Cogeneration Plant in the Distillation Phase.

Cutzu, R. & Bardi, L. (2017), 3(2), 24.

Improvement of the protein quality of wheat bread through faba bean sourdough addition.

Coda, R., Varis, J., Verni, M., Rizzello, C. G. & Katina, K. (2017). LWT-Food Science and Technology, 82, 296-302.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.