D-Glucosamine Assay Kit

The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products.

Suitable for manual, auto-analyser and microplate formats.

Product Code
Content/size
Stock
Price
Qty
K-GAMINE
50 assays (manual) / 500 assays (microplate)
/ 500 assays (auto-analyser)
$206.00

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UV-method for the determination of D-Glucosamine,
D-Glucosamine sulphate and N-Acetyl Glucosamine in food
supplements, foodstuffs, beverages and other materials

Principle:
                                     (desulphation)
(1) Glucosamine sulphate → D-glucosamine + sulphate

                                   (deacetylation)
(2) N-Acetyl Glucosamine → D-glucosamine + acetate

                                    (hexokinase)
(3) D-Glucosamine + ATP → D-glucosamine-6-P + ATP

                   (glucosamine 6-phosphate deaminase)
(4) D-Glucosamine-6-P + H2O → D-fructose-6-P + NH4+

    (phosphoglucose isomerase)
(5)  F-6-P             →            G-6-P

     (glucose-6-phosphate dehydrogenase)
(6) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                             * 50 assays (manual) / 500 (microplate)
                                           / 500 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                             Spectrophotometric at 340 nm
Reaction time:                   ~ 8 min
Detection limit:                 1.33 mg/L
Application examples:
Food supplements, food products and beverages
Method recognition:     Novel method

Advantages

  • Novel product with simple format
     
  • All reagents stable for > 2 years after preparation
     
  • All enzymes supplied as stable suspensions
     
  • Very rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Glucosamine inhibits decidualization of human endometrial stromal cells and decreases litter sizes in mice.

Tsai, J. H., Schulte, M., O'Neill, K., Chi, M. M. Y., Frolova, A. I. & Moley, K. H. (2013). Biology of Reproduction, 89(1), 16, 1-10.

Cuticle hydrolysis in four medically important fly species by enzymes of the entomopathogenic fungus Conidiobolus coronatus.

Boguś, M. I., Włóka, E., Wrońska, A., Kaczmarek, A., Kazek, M., Zalewska, K., Ligeza-Zuber, M. & Gołębiowski, M. (2016). Medical and Veterinary Entomology, 31(1), 23-35.

Extraction of Glycosaminoglycans Containing Glucosamine and Chondroitin Sulfate from Chicken Claw Cartilage.

Widyaningsih, T. D., Rukmi, W. D., Sofia, E., Wijayanti, S. D., Wijayanti, N., Ersalia, R., Rochmawati, N. & Nangin, D. (2016). Research Journal of Life Science, 3(3), 181-189.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs