D-Glucose HK Assay Kit 

The D-Glucose HK (Regular) test kit is a high purity reagent for the measurement and analysis of D-glucose in plant and food products. Can be used in combination with other Megazyme's products that require glucose determination.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

Product Code
220 assays (manual) / 2200 (microplate) / 2000 (auto-analyser)
110 assays (manual) / 1100 (microplate) / 1000 (auto-analyser)

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UV-method for the determination of D-Glucose in foodstuffs,
beverages and other materials

(1) D-Glucose + ATP → G-6-P + ADP

      (glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                            (K-GLUHK-110A)
110 assays (manual) / 1100 (microplate)
                                            / 1000 (auto-analyser) or
220 assays (manual) / 2200 (microplate)
                                            / 2000 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 5 min
Detection limit:                  0.66 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals
(e.g. infusions), feed, paper (and cardboard) and other materials (e.g.
biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by AOAC, EN,


  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

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Grape and wine analysis: Oenologists to exploit advanced test kits.

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Megazyme “advanced” wine test kits general characteristics and validation.

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Measurement of carbohydrates in grain, feed and food.

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Measurement of Starch: Critical evaluation of current methodology.

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Advances in molecular tools for the use of Zygosaccharomyces bailii as host for biotechnological productions and construction of the first auxotrophic mutant.

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Influence of different carbon sources on bacterial cellulose production by Gluconacetobacter xylinus strain ATCC 53524.

Mikkelsen, D., Flanagan, B. M., Dykes, G. A. & Gidley, M. J. (2009). Journal of Applied Microbiology, 107(2), 576-583.

Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus.

Centeno, M. S., Guerreiro, C. I. P. D., Dias, F. M.V., Morland, C., Tailford, L. E., Goyal, A., Prates, J. A. M., Ferreira, L. M. A., Caldeira, R. M. H., Mongodin, E. F., Nelson, K. E., Gilbert, H. J. & Fontes, C. M. (2006). FEMS Microbiology Letters, 261(1), 123-132.

Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations.

Ödman, P., Johansen, C. L., Olsson, L., Gernaey, K. V. & Lantz, A. E. (2010). Applied Microbiology and Biotechnology, 86(6), 1745-1759.

Sealing ability of a new polydimethylsiloxane-based root canal filling material.

Özok, A. R., van der Sluis, L. W. M., Wu, M. K. & Wesselink, P. R. (2008). Journal of Endodontics, 34(2), 204-207.

Adipose triglyceride lipase plays a key role in the supply of the working muscle with fatty acids.

Schoiswohl, G., Schweiger, M., Schreiber, R., Gorkiewicz, G., Preiss-Landl, K., Taschler, U., Zierler, K. A., Radner, F. P. W., Eichmann, T. O., Kienesberger, P. C., Eder, S., Lass, A., Haemmerle, G., Alsted, T. J., Kiens, B., Hoefler, G., Zechner, R. & Zimmermann, R. (2010). Journal of Lipid Research, 51(3), 490-499.

Experimental evidence for a significant contribution of cellulose to indoor aerosol mass concentration.

Cerqueira, M., Marques, D., Caseiro, A. & Pio, C. (2010). Atmospheric Environment, 44(6), 867-871.

Effect of HXT1 and HXT7 hexose transporter overexpression on wild-type and lactic acid producing Saccharomyces cerevisiae cells.

Rossi, G., Sauer, M., Porro, D. & Branduardi, P. (2010). Microbial Cell Factories, 9(1), 15.

Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants.

Jönsson, M., Saleihan, Z., Nes, I. F. & Holo, H. (2009). Applied and Environmental Microbiology, 75(14), 4901-4903.

Preloaded Tissues for Descemet Membrane Endothelial Keratoplasty.

Parekh, M., Ruzza, A., Ferrari, S., Busin, M. & Ponzin, D. (2016). American Journal of Ophthalmology, 166, 120-125.

Characterization of the isolated active layer of a NF membrane by electrochemical impedance spectroscopy.

Efligenir, A., Fievet, P., Déon, S. & Salut, R. (2015). Journal of Membrane Science, 477, 172-182.

Interaction mechanism between green tea extract and human α-amylase for reducing starch digestion.

Miao, M., Jiang, B., Jiang, H., Zhang, T. & Li, X. (2015). Food Chemistry, 186, 20-25.

Spatial reorganization of telomeres in long-lived quiescent cells.

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Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment.

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