D-Glucose HK Assay Kit 

The D-Glucose HK (Regular) test kit is a high purity reagent for the measurement and analysis of D-glucose in plant and food products. Can be used in combination with other Megazyme's products that require glucose determination.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

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Product Code
Content/Size
Stock
Price
Qty
K-GLUHK-220A
220 assays (manual) / 2200 (microplate) / 2000 (auto-analyser)
$324.00
K-GLUHK-110A
110 assays (manual) / 1100 (microplate) / 1000 (auto-analyser)
$183.00

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UV-method for the determination of D-Glucose in foodstuffs,
beverages and other materials

Principle:
                          (hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

      (glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                            (K-GLUHK-110A)
                                           110 assays (manual) / 1100 (microplate)
                                            / 1000 (auto-analyser) or
                                           (K-GLUHK-220A)
                                           220 assays (manual) / 2200 (microplate)
                                            / 2000 (auto-analyser)
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 5 min
Detection limit:                  0.66 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals
(e.g. infusions), feed, paper (and cardboard) and other materials (e.g.
biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years after preparation
     
  • Rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Extended cofactors stability
     
  • Suitable for manual, microplate and auto-analyser formats

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

Advances in molecular tools for the use of Zygosaccharomyces bailii as host for biotechnological productions and construction of the first auxotrophic mutant.

Dato, L., Branduardi, P., Passolunghi, S., Cattaneo, D., Riboldi, L., Frascotti, G., Valli, M. & Porro, D. (2010). FEMS Yeast Research, 10(7), 894-908.

Influence of different carbon sources on bacterial cellulose production by Gluconacetobacter xylinus strain ATCC 53524.

Mikkelsen, D., Flanagan, B. M., Dykes, G. A. & Gidley, M. J. (2009). Journal of Applied Microbiology, 107(2), 576-583.

Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus.

Centeno, M. S., Guerreiro, C. I. P. D., Dias, F. M.V., Morland, C., Tailford, L. E., Goyal, A., Prates, J. A. M., Ferreira, L. M. A., Caldeira, R. M. H., Mongodin, E. F., Nelson, K. E., Gilbert, H. J. & Fontes, C. M. (2006). FEMS Microbiology Letters, 261(1), 123-132.

Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations.

Ödman, P., Johansen, C. L., Olsson, L., Gernaey, K. V. & Lantz, A. E. (2010). Applied Microbiology and Biotechnology, 86(6), 1745-1759.

Sealing ability of a new polydimethylsiloxane-based root canal filling material.

Özok, A. R., van der Sluis, L. W. M., Wu, M. K. & Wesselink, P. R. (2008). Journal of Endodontics, 34(2), 204-207.

Adipose triglyceride lipase plays a key role in the supply of the working muscle with fatty acids.

Schoiswohl, G., Schweiger, M., Schreiber, R., Gorkiewicz, G., Preiss-Landl, K., Taschler, U., Zierler, K. A., Radner, F. P. W., Eichmann, T. O., Kienesberger, P. C., Eder, S., Lass, A., Haemmerle, G., Alsted, T. J., Kiens, B., Hoefler, G., Zechner, R. & Zimmermann, R. (2010). Journal of Lipid Research, 51(3), 490-499.

Experimental evidence for a significant contribution of cellulose to indoor aerosol mass concentration.

Cerqueira, M., Marques, D., Caseiro, A. & Pio, C. (2010). Atmospheric Environment, 44(6), 867-871.

Effect of HXT1 and HXT7 hexose transporter overexpression on wild-type and lactic acid producing Saccharomyces cerevisiae cells.

Rossi, G., Sauer, M., Porro, D. & Branduardi, P. (2010). Microbial Cell Factories, 9(1), 15.

Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants.

Jönsson, M., Saleihan, Z., Nes, I. F. & Holo, H. (2009). Applied and Environmental Microbiology, 75(14), 4901-4903.
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs