D-Glucuronic/D-Galacturonic Acid Assay Kit

The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically
D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.

Suitable for manual, auto-analyser and microplate formats.

Product Code
100 assays (manual) / 1000 assays (microplate)
/ 1000 assays (auto-analyser)

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UV-method for the determination of D-Glucuronic Acid or
D-Galacturonic Acid in hydrolysates of plant material and
polysaccharides and other materials

                                       (Uronate dehydrogenase; UDH)
(1) D-Glucuronic acid + NAD+ + H2O → D-glucarate + NADH + H+

                                         (Uronate dehydrogenase; UDH)
(2) D-Galacturonic acid + NAD+ + H2O → D-galactarate + NADH + H+

Kit size:                            100 assays (manual) / 1000 (microplate)
                                          / 1000 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 10 min at 25°C or ~ 5 min at 37°C
Detection limit:                 ~ 17 mg/L
Application examples:
Hydrolysates of plant material and polysaccharides and other
Method recognition:         Novel method


  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only test kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling.

Li, M., Pattathil, S., Hahn, M. G. & Hodge, D. B. (2014). RSC Advances, 4(33), 17282-17292.

Correlating lignin structural features to phase partitioning behavior in a novel aqueous fractionation of softwood Kraft black liquor.

Stoklosa, R. J., Velez, J., Kelkar, S., Saffron, C. M., Thies, M. C. & Hodge, D. B. (2013). Green Chemistry, 15(10), 2904-2912.

Dietary Supplementation with Soluble Plantain Non-Starch Polysaccharides Inhibits Intestinal Invasion of Salmonella Typhimurium in the Chicken.

Parsons, B. N., Wigley, P., Simpson, H. L., Williams, J. M., Humphrey, S., Salisbury, A. M., Watson, A. J., Fry, S. C., O'Brien, D., Roberts, C. L., O'Kennedy, N., Keita, Å. V., Söderholm, J. D., Rhodes, J. M. & Campbell, B. J. (2014). PloS One, 9(2), e87658.

Optimization of a formic/acetic acid delignification treatment on beech wood and its influence on the structural characteristics of the extracted lignins.

Simon, M., Brostaux, Y., Vanderghem, C., Jourez, B., Paquot, M. & Richel, A. (2014). Journal of Chemical Technology and Biotechnology, 89(1), 128-136.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.