D-Glucuronic/D-Galacturonic Acid Assay Kit

The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically
D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.

Suitable for manual, auto-analyser and microplate formats.

Product Code
Content/size
Stock
Price
Qty
K-URONIC
100 assays (manual) / 1000 assays (microplate)
/ 1000 assays (auto-analyser)
$258.00

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UV-method for the determination of D-Glucuronic Acid or
D-Galacturonic Acid in hydrolysates of plant material and
polysaccharides and other materials

Principle:
                                       (Uronate dehydrogenase; UDH)
(1) D-Glucuronic acid + NAD+ + H2O → D-glucarate + NADH + H+

                                         (Uronate dehydrogenase; UDH)
(2) D-Galacturonic acid + NAD+ + H2O → D-galactarate + NADH + H+

Kit size:                             * 100 assays (manual) / 1000 (microplate)
                                          / 1000 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                             Spectrophotometric at 340 nm
Reaction time:                  ~ 10 min at 25°C or ~ 5 min at 37°C
Detection limit:                 ~ 17 mg/L
Application examples:
Hydrolysates of plant material and polysaccharides and other
materials
Method recognition:   Novel method

Advantages

  • Very cost effective
     
  • All reagents stable for > 2 years during use
     
  • Only test kit available
     
  • Simple format
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling.

Li, M., Pattathil, S., Hahn, M. G. & Hodge, D. B. (2014). RSC Advances, 4(33), 17282-17292.

Correlating lignin structural features to phase partitioning behavior in a novel aqueous fractionation of softwood Kraft black liquor.

Stoklosa, R. J., Velez, J., Kelkar, S., Saffron, C. M., Thies, M. C. & Hodge, D. B. (2013). Green Chemistry, 15(10), 2904-2912.

Dietary Supplementation with Soluble Plantain Non-Starch Polysaccharides Inhibits Intestinal Invasion of Salmonella Typhimurium in the Chicken.

Parsons, B. N., Wigley, P., Simpson, H. L., Williams, J. M., Humphrey, S., Salisbury, A. M., Watson, A. J., Fry, S. C., O'Brien, D., Roberts, C. L., O'Kennedy, N., Keita, Å. V., Söderholm, J. D., Rhodes, J. M. & Campbell, B. J. (2014). PloS One, 9(2), e87658.

Optimization of a formic/acetic acid delignification treatment on beech wood and its influence on the structural characteristics of the extracted lignins.

Simon, M., Brostaux, Y., Vanderghem, C., Jourez, B., Paquot, M. & Richel, A. (2014). Journal of Chemical Technology and Biotechnology, 89(1), 128-136.

Fractionation and improved enzymatic deconstruction of hardwoods with alkaline delignification.

Stoklosa, R. J. & Hodge, D. B. (2015). BioEnergy Research, 8(3), 1224-1234.

Kinetic properties of Rhizopus oryzae RPG1 endo-polygalacturonase hydrolyzing galacturonic acid oligomers.

Mertens, J. A. & Bowman, M. J. (2016). Biocatalysis and Agricultural Biotechnology, 5, 11-16.

Characterisation of three fungal glucuronoyl esterases on glucuronic acid ester model compounds.

Hüttner, S., Klaubauf, S., de Vries, R. P. & Olsson, L. (2017). Applied Microbiology and Biotechnology, 1-11.

Action of a GH115 α-glucuronidase from Amphibacillus xylanus at alkaline condition promotes release of 4-O -methylglucopyranosyluronic acid from glucuronoxylan and arabinoglucuronoxylan.

Yan, R., Vuong, T. V., Wang, W. & Master, E. R. (2017). Enzyme and Microbial Technology, In press.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs