D-Isocitric Acid Assay Kit

The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.

Suitable for manual, auto-analyser and microplate formats.

Product Code
100 assays (manual) / 1000 assays (microplate)
/ 1000 assays (auto-analyser)

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UV-method for the determination of D-Isocitric Acid in foodstuffs

                            (isocitrate dehydrogenase)
(1) D-Isocitric acid + NADP+ → 2-oxoglutarate + CO2 + NADPH + H+

                                          (pH 9-10)
(2) D-Isocitric acid ester + H2O → D-isocitric acid + alcohol

                                              (pH 9-10)
(3) D-Isocitric acid lactone + H2O → D-isocitric acid

Kit size:    100 assays (manual) / 1000 (microplate)
                                          / 1000 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 3 min
Detection limit:                 0.35 mg/L
Application examples:
Fruit juices, fruit products, soft drinks and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by EN, NEN, NF,


  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • No wasted isocitrate dehydrogenase solution (stable suspension supplied)
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Metabolic Flexibility of Yarrowia lipolytica Growing on Glycerol.

Egermeier, M., Russmayer, H., Sauer, M. & Marx, H. (2017). Frontiers in Microbiology, 8(49).

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.