D-Lactate dehydrogenase (Leuconostoc mesenteroides

High purity recombinant D-Lactate dehydrogenase (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 1.1.1.28
CAS: 9028-36-8

D-lactate dehydrogenase; (R)-lactate:NAD+ oxidoreductase.

Recombinant. From Leuconostoc mesenteroides.
In 3.2 M ammonium sulphate.
Supplied at ~ 20,000 U/mL. 

Specific activity:
~2,085 U/mg (25oC, pH 7.0 on pyruvic acid).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-DLDHLM
22,000 Units
$158.00

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DESCRIPTION

D-Lactate dehydrogenase (Leuconostoc mesenteroides)

EC 1.1.1.28
CAS: 9028-36-8

Synonyms:
D-lactate dehydrogenase; (R)-lactate:NAD+ oxidoreductase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~2,085 U/mg (25oC, pH 7.0 on pyruvic acid).

Unit definition:
One Unit of D-lactate dehydrogenase is defined as the amount of enzyme required to produce one μmole of D-lactate from pyruvic acid (0.77 mM) per minute in the presence of NADH in sodium phosphate buffer (92 mM), pH 7.0 at 25oC.

Specificity:
Catalyses the reaction:
D-Lactate + NAD+ = pyruvate + NADH + H+

Applications:
Applications for the measurement of D-lactic acid in the food, fermentation, wine, beverage and dairy industries.

Metabolic response of glioblastoma cells associated with glucose withdrawal and pyruvate substitution as revealed by GC-MS.

Oppermann, H., Ding, Y., Sharma, J., Paetz, M. B., Meixensberger, J., Gaunitz, F. & Birkemeyer, C. (2016). Nutrition & Metabolism, 13(1), 70.