D-Mannitol/L-Arabitol Assay Kit

The D-Mannitol/L-Arabitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol and L-arabitol in food, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

Data calculators are located in the Documentation tab.

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Product Code
60 assays (manual) / 600 assays (microplate)
/ 580 assays (auto-analyser)

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UV-method for the determination of D-Mannitol and L-Arabitol
in foodstuffs and other materials

                    (mannitol dehydrogenase)
(1) D-Mannitol + NAD+ → D-fructose + NADH + H+

                  (mannitol dehydrogenase)
(2) L-Arabitol + NAD+ → L-xylulose + NADH + H+

Kit size:                             60 assays (manual) / 600 (microplate)
                                           / 580 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 4 min
Detection limit:                 0.50 mg/L
Application examples:
Wine, chewing gum, dietetic foods, candies, cosmetics, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:        Novel method


  • Novel product (only enzymatic kit available)
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

In vitro and in vivo protective effects of fermented preparations of dietary herbs against lipopolysaccharide insult.

Bose, S., Song, M. Y., Nam, J. K., Lee, M. J. & Kim, H. (2012). Food Chemistry, 134(2), 758-765.

Botrytis cinerea isolates collected from grapes present different requirements for conidia germination.

Cotoras, M., Garcia, C. & Mendoza, L. (2009). Mycologia, 101(3), 287-295.

The effects of co-administration of probiotics with herbal medicine on obesity, metabolic endotoxemia and dysbiosis: A randomized double-blind controlled clinical trial.

Lee, S. J., Bose, S., Seo, J. G., Chung, W. S., Lim, C. Y. & Kim, H. (2013). Clinical Nutrition, 33(6), 973-981.

Engineering of photosynthetic mannitol biosynthesis from CO2 in a cyanobacterium.

Jacobsen, J. H. & Frigaard, N. U. (2014). Metabolic Engineering, 21, 60-70.
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.