D-Mannitol/L-Arabitol Assay Kit

The D-Mannitol/L-Arabitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol and L-arabitol in food, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

Data calculators are located in the Documentation tab.

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Product Code
Content/size
Stock
Price
Qty
K-MANOL
60 assays (manual) / 600 assays (microplate)
/ 580 assays (auto-analyser)
$270.00

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UV-method for the determination of D-Mannitol and L-Arabitol
in foodstuffs and other materials

Principle:
                    (mannitol dehydrogenase)
(1) D-Mannitol + NAD+ → D-fructose + NADH + H+

                  (mannitol dehydrogenase)
(2) L-Arabitol + NAD+ → L-xylulose + NADH + H+

Kit size:                             * 60 assays (manual) / 600 (microplate)
                                           / 580 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 4 min
Detection limit:                 0.50 mg/L
Application examples:
Wine, chewing gum, dietetic foods, candies, cosmetics, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    Novel method

Advantages

  • Novel product (only enzymatic kit available)
     
  • Very cost effective
     
  • All reagents stable for > 2 years after preparation
     
  • Simple format
     
  • Rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Extended cofactors stability
     
  • Suitable for manual, microplate and auto-analyser formats

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

In vitro and in vivo protective effects of fermented preparations of dietary herbs against lipopolysaccharide insult.

Bose, S., Song, M. Y., Nam, J. K., Lee, M. J. & Kim, H. (2012). Food Chemistry, 134(2), 758-765.

Botrytis cinerea isolates collected from grapes present different requirements for conidia germination.

Cotoras, M., Garcia, C. & Mendoza, L. (2009). Mycologia, 101(3), 287-295.

The effects of co-administration of probiotics with herbal medicine on obesity, metabolic endotoxemia and dysbiosis: A randomized double-blind controlled clinical trial.

Lee, S. J., Bose, S., Seo, J. G., Chung, W. S., Lim, C. Y. & Kim, H. (2013). Clinical Nutrition, 33(6), 973-981.

Engineering of photosynthetic mannitol biosynthesis from CO2 in a cyanobacterium.

Jacobsen, J. H. & Frigaard, N. U. (2014). Metabolic Engineering, 21, 60-70.

Hepatoprotective effect of Lentinus edodes mycelia fermented formulation against alcoholic liver injury in rats.

Chung, W. S., Wang, J. H., Bose, S., Park, J. M., Park, S. O., Lee, S. J., Jeon. S. & Kim, H. (2015). Journal of Food Biochemistry, 39(3), 251-262.

Strategic optimization of xylanase–mannanase combi-CLEAs for synergistic and efficient hydrolysis of complex lignocellulosic substrates.

Bhattacharya, A. & Pletschke, B. I. (2015). Journal of Molecular Catalysis B: Enzymatic, 115, 140-150.
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs