D-Mannose/D-Fructose/D-Glucose Assay kit

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

Product Code
55 assays per kit

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UV-method for the determination of D-Mannose, D-Fructose
and D-Glucose in foodstuffs, yeast cell preparations and
other materials

(1) D-Mannose / D-fructose / D-glucose + ATP →
                                                           M-6-P / F-6-P / G-6-P + ADP

     (glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

    (phosphomannose isomerase) (phosphoglucose isomerase)
(3)  M-6-P              ↔              F-6-P              ↔              G-6-P

Kit size:                             * 55 assays

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 30 min
Detection limit:                 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition:        Novel method


  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Altered physiology and biochemistry of imported litchi fruit held under different vapor pressure deficits.

Somboonkaew, N. & Terry, L. A. (2010). Journal of Agricultural and Food Chemistry, 58(10), 6209-6218.

A new bacterial hydrolase specific for the compatible solutes α-D-mannopyranosyl-(1→2)-D-glycerate and α-D-glucopyranosyl-(1→2)-D-glycerate.

Alarico, S., Empadinhas, N. & da Costa, M. S. (2013). Enzyme and Microbial Technology, 52(2), 77-83.

The molecular characterization of a novel GH38 α-mannosidase from the crenarchaeon Sulfolobus solfataricus revealed its ability in de-mannosylating glycoproteins.

Cobucci-Ponzano, B., Conte, F., Strazzulli, A., Capasso, C., Fiume, I., Pocsfalvi, G., Rossi, M. & Moracci, M. (2010). Biochimie, 92(12), 1895-1907.

The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate.

Nobre, A., Empadinhas, N., Nobre, M. F., Lourenço, E. C., Maycock, C., Ventura, M. R., Mingote A. & da Costa, M. S. (2013). Planta, 237(3), 891-901.

Cell lysis induced by membrane-damaging detergent saponins from Quillaja saponaria.

Berlowska, J., Dudkiewicz, M., Kregiel, D., Czyzowska, A. & Witonska, I. (2015). Enzyme and Microbial Technology, 75, 44-48.

Strategic optimization of xylanase–mannanase combi-CLEAs for synergistic and efficient hydrolysis of complex lignocellulosic substrates.

Bhattacharya, A. & Pletschke, B. I. (2015). Journal of Molecular Catalysis B: Enzymatic, 115, 140-150.

A Bacteroidetes locus dedicated to fungal 1,6-β-glucan degradation: unique substrate conformation drives specificity of the key endo-1,6-β-glucanase.

Temple, M. J., Cuskin, F., Baslé, A., Hickey, N., Speciale, G., Williams, S. J., Gilbert, H. J. & Lowe, E. C. (2017). Journal of Biological Chemistry, jbc-M117.

Use of almond shell as food ingredient.

Kacem, I., Martinez-Saez, N., Kallel, F., Khawla, J. B., Claire, H. B., Semia, C. E. & del Castillo, M. D. (2017). European Food Research and Technology, 1-12.

The carbon consumption pattern of the spoilage yeast Brettanomyces bruxellensis in synthetic wine-like medium.

Smith, B. D. & Divol, B. (2017). Food Microbiology, 73, 39-48.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.