D-Mannose/D-Fructose/D-Glucose Assay kit

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

Product Code
Content/size
Stock
Price
Qty
K-MANGL
55 assays per kit
$258.00

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UV-method for the determination of D-Mannose, D-Fructose
and D-Glucose in foodstuffs, yeast cell preparations and
other materials

Principle:
                                                                 (hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
                                                           M-6-P / F-6-P / G-6-P + ADP

     (glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

    (phosphomannose isomerase) (phosphoglucose isomerase)
(3)  M-6-P              ↔              F-6-P              ↔              G-6-P

Kit size:                             55 assays
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 30 min
Detection limit:                 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition:        Novel method

Advantages

  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years after preparation
     
  • Only enzymatic kit available
     
  • Simple format
     
  • Rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

Altered physiology and biochemistry of imported litchi fruit held under different vapor pressure deficits.

Somboonkaew, N. & Terry, L. A. (2010). Journal of Agricultural and Food Chemistry, 58(10), 6209-6218.

A new bacterial hydrolase specific for the compatible solutes α-D-mannopyranosyl-(1→2)-D-glycerate and α-D-glucopyranosyl-(1→2)-D-glycerate.

Alarico, S., Empadinhas, N. & da Costa, M. S. (2013). Enzyme and Microbial Technology, 52(2), 77-83.

The molecular characterization of a novel GH38 α-mannosidase from the crenarchaeon Sulfolobus solfataricus revealed its ability in de-mannosylating glycoproteins.

Cobucci-Ponzano, B., Conte, F., Strazzulli, A., Capasso, C., Fiume, I., Pocsfalvi, G., Rossi, M. & Moracci, M. (2010). Biochimie, 92(12), 1895-1907.

The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate.

Nobre, A., Empadinhas, N., Nobre, M. F., Lourenço, E. C., Maycock, C., Ventura, M. R., Mingote A. & da Costa, M. S. (2013). Planta, 237(3), 891-901.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs