D-Sorbitol/Xylitol Assay Kit

The D-Sorbitol/Xylitol test kit is suitable for the measurement and analysis of D-sorbitol and/or xylitol in food products.

Suitable for manual, auto-analyser and microplate formats.

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Product Code
Content/size
Stock
Price
Qty
K-SORB
58 assays (manual) / 580 assays (microplate)
/ 700 assays (auto-analyser)
$247.00

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Colourimetric method for the determination of D-Sorbitol and
Xylitol in foodstuffs and wine

Principle:
                      (sorbitol dehydrogenase)
(1) D-Sorbitol + NAD+ ↔ D-fructose + NADH + H+

               (sorbitol dehydrogenase)
(2) Xylitol + NAD+ ↔ D-xylulose + NADH + H+

                         (diaphorase)
(3) INT + NADH + H+ → NAD+ + INT-formazan

Kit size:                             * 58 assays (manual) / 580 (microplate)
                                           / 700 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 492 nm
Reaction time:                  ~ 15 min
Detection limit:                 0.20 mg/L
Application examples:
Diabetic foods (e.g. honey, jam and chocolate), dietetic foods,
chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets,
bakery products (e.g. desserts), marzipan, paper (and cardboard),
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by IFU and AIJN

Advantages

  • Each vial of sorbitol dehydrogenase is stable for > 2 months at 4°C after dissolution
     
  • No wasted diaphorase solution (stable suspension supplied)
     
  • Very competitive price (cost per test)
     
  • Reagents stable for > 2 years as supplied
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Aldose reductase is implicated in high glucose‐induced oxidative stress in mouse embryonic neural stem cells.

Fu, J., Tay, S. S. W., Ling, E. A. & Dheen, S. T. (2007). Journal of Neurochemistry, 103(4), 1654-1665.

Assessment of xylitol serum levels during the course of parenteral nutrition including xylitol in intensive care patients: A case control study.

Schneider, A. S., Schettler, A., Markowski, A., Luettig, B., Momma, M., Seipt, C., Hadem, J. & Wilhelmi, M. (2014). Clinical Nutrition, 33(3), 483-488.

The peach (Prunus persica [L.] Batsch) homeobox gene KNOPE3, which encodes a class 2 knotted-like transcription factor, is regulated during leaf development and triggered by sugars.

Testone, G., Condello, E., Verde, I., Caboni, E., Iannelli, M. A., Bruno, L., Mariotti, D., Bitonti, M. B. & Giannino, D. (2009). Molecular Genetics and Genomics, 282(1), 47-64.

A novel aldose-aldose oxidoreductase for co-production of D-xylonate and xylitol from D-xylose with Saccharomyces cerevisiae.

Wiebe, M. G., Nygård, Y., Oja, M., Andberg, M., Ruohonen, L., Koivula, A., Penttilä, M. & Toivari, M. (2015). Applied Microbiology and Biotechnology, 99(22), 9439-9447.
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs