Diaphorase (E. coli

High purity recombinant Diaphorase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 1.8.1.4
CAS: 9001-18-7 

dihydrolipoyl dehydrogenase; protein-N6-(dihydrolipoyl)lysine:NAD+ oxidoreductase

Recombinant. From Escherichia coli.
In 3.2 M ammonium sulphate.
Supplied at ~ 275 U/mL. 

Specific activity:
~ 12 U/mg (25oC, pH 9.0). 

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-DIAEC
1,000 Units
$224.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

Diaphorase (Escherichia coli)

EC 1.8.1.4
CAS: 9001-18-7

Synonyms:
dihydrolipoyl dehydrogenase; protein-N6-(dihydrolipoyl)lysine:NADoxidoreductase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 12 U/mg (25oC, pH 9.0).

Unit definition:
One Unit of diaphorase activity is defined as the amount of enzyme required to oxidise one μmole of NADH per minute with associated reduction of INT in Tris.HCl buffer (32.3 mM), pH 9.0 at 25oC.

Specificity:
Catalyses the reaction:
Tetrazolium compound + NAD+ →  formazan compound + NADH + H+

Applications:
Used widely in diagnostic and analytical applications.