Feruloyl esterase (rumen microorganism) 

High purity recombinant Feruloyl Esterase (rumen microorganism) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.1.1.73
CAZy Family: CE1
CAS: 134712-49-5/224306-54-1/224306-55-2

feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase

Recombinant. From rumen microorganism.
In 3.2 M ammonium sulphate.
Supplied at ~ 600 U/mL. 

Specific activity:
~ 40 U/mg (40oC, pH 6.5 on ethyl ferulate).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-FAERU
1,000 Units
$185.00

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DESCRIPTION

Feruloyl esterase (rumen microorganism)

EC 3.1.1.73
CAZy Family: CE1
CAS: 134712-49-5/224306-54-1/224306-55-2

Synonyms:
feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 40 U/mg (40oC, pH 6.5 on ethyl ferulate).

Unit definition:
One Unit of feruloyl esterase activity is defined as the amount of enzyme required to release one μmole of ferulic acid per minute from ethyl-ferulate (0.39 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC.

Specificity:
Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates.

Applications:
Applications established in biofuels, paper and pulp, food, nutrition, medical and pharmacological industries.

The role of activity coefficients in bioreaction equilibria: Thermodynamics of methyl ferulate hydrolysis.

Hoffmann, P., Voges, M., Held, C. & Sadowski, G. (2013). Biophysical Chemistry, 173, 21-30.