Feruloyl esterase (rumen microorganism) 

High purity recombinant Feruloyl Esterase (rumen microorganism) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: CE1
CAS: 134712-49-5/224306-54-1/224306-55-2

feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase

Recombinant. From rumen microorganism.
In 3.2 M ammonium sulphate.
Supplied at ~ 400 U/mL. 

Specific activity:
~ 30 U/mg (40oC, pH 6.5 on ethyl ferulate).

StabilityMinimum 1 year at 4oC. Check vial for details.

Product Code
1,000 Units

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Feruloyl esterase (rumen microorganism)

CAZy Family: CE1
CAS: 134712-49-5/224306-54-1/224306-55-2

feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase

In 3.2 M ammonium sulphate.

Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 30 U/mg (40oC, pH 6.5 on ethyl ferulate).

Unit definition:
One Unit of feruloyl esterase activity is defined as the amount of enzyme required to release one μmole of ferulic acid per minute from ethyl-ferulate (0.39 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC.

Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates.

Applications established in biofuels, paper and pulp, food, nutrition, medical and pharmacological industries.

The role of activity coefficients in bioreaction equilibria: Thermodynamics of methyl ferulate hydrolysis.

Hoffmann, P., Voges, M., Held, C. & Sadowski, G. (2013). Biophysical Chemistry, 173, 21-30.