Free Sulfite Assay Kit

The Free Sulphite test kit is a rapid, simple, reliable and accurate method for the measurement and analysis of free sulfite (sulphite) in wine, beverages, food stuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual, auto-analyser and microplate formats.

Suitable for manual, auto-analyser and microplate formats.

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Product Code
80 assays (manual) / 800 assays (microplate)
/ 800 assays (auto-analyser)

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Colourimetric method for the determination of Free Sulfite 
in wine, fruit juice, foodstuffs and other materials

The Free Sulfite assay is based on the reaction principle of SO2, fuchsin and aldehyde binding

Kit size:                              80 assays (manual) / 800 (microplate)
                                            / 800 (auto-analyser)
Method:                              Free sulfite: Spectrophotometric at 575 nm
Total assay time:              Free sulfite: ~ 9 min
Detection limit:                 Free sulfite: ~ 2 mg/L
Application examples:
Wine, fruit juice, sea food, food stuffs and other materials
Method recognition:    
Validated for red and white wines at the Bundesamt für Weinbau, Austria.
Used widely in the wine industry


  • ”Ready to use" liquid stable Formulation 
  • Very competitive price (cost per test) 
  • All reagents stable for > 18 months 
  • Very rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included 
  • Suitable for manual, microplate and auto-analyser formats
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction & Kit Description
Chapter 2: Theory of Assay Procedure
Chapter 3: Standards & Calibration Curves
Chapter 4: Preparation of the Single Point Standard
Chapter 5: Preparation of the Calibration Curve
Chapter 6: Assay Procedure
Chapter 7: Free Sulphite Calculations

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.