Galactomannan Assay Kit

The Galactomannon test kit is suitable for the measurement and analysis of Galactomannan in food and plant products.

Product Code
100 Assays per kit

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UV-method for the determination of Galactomannan in legume
seeds, foodstuffs and plant products

(1) Galactomannan + H2O → galactomanno-oligomers

                                  (β-mannanase + α-galactosidase)
(2) Galactomanno-oligomers + H2O → D-galactose +

                   (β-galactose dehydrogenase)
(3) D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Kit size:                             100 assays
Method:                            Spectrophotometric at 340 nm
Total assay time:              ~ 90 min
Detection limit:                 1-100% of sample weight
Application examples:
Seeds, milling fractions and food ingredients
Method recognition:        Novel method


  • Galactose dehydrogenase now included in the kit
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

An enzymic technique for the quantitation of galactomannan in guar Seeds.

McCleary, B. V. (1981). Lebensmittel-Wissenschaft & Technologie, 14, 56-59.

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

Metabolic and genetic perturbations accompany the modification of galactomannan in seeds of Medicago truncatula expressing mannan synthase from guar (Cyamopsis tetragonoloba L.).

Naoumkina, M., Vaghchhipawala, S., Tang, Y., Ben, Y., Powell, R. J. & Dixon, R. A. (2008). Plant Biotechnology Journal, 6(6), 619-631.

Enzymatic improvement of guar‐based thickener for better‐quality silk screen printing.

Baldaro, E., Gallucci, M., Formantici, C., Issi, L., Cheroni, S. & Galante, Y. M. (2012). Coloration Technology, 128(4), 315-322.

Nutrient utilisation and intestinal fermentation are differentially affected by the consumption of resistant starch varieties and conventional fibres in pigs.

Rideout, T. C., Liu, Q., Wood, P. & Fan, M. Z. (2008). British Journal of Nutrition, 99(05), 984-992.

Seed yield, galactomannan content and quality traits of different guar (Cyamopsis tetragonoloba L.) genotypes.

Gresta, F., Ceravolo, G., Presti, V. L., D’Agata, A., Rao, R. & Chiofalo, B. (2017). Industrial Crops and Products, 107, 122-129.

Galactomannan from Trigonella foenum‐graecum L. seed: Prebiotic application and its fermentation by the probiotic Bacillus coagulans strain MTCC 5856.

Majeed, M., Majeed, S., Nagabhushanam, K., Arumugam, S., Natarajan, S., Beede, K. & Ali, F. (2018). Food Science & Nutrition, In Press.

Lipopolysaccharide immune stimulation but not β-mannanase supplementation affects maintenance energy requirements in young weaned pigs.

Huntley, N. F., Nyachoti, C. M. & Patience, J. F. (2018). Journal of Animal Science and Biotechnology, 9(1), 47.

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