Galactose dehydrogenase - Galactose mutarotase 

High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.

EC 1.1.1.48 (Galactose dehydrogenase)
CAS: 9028-54-0

D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase

EC 5.1.3.3 (Galactose mutarotase)
CAS: 9031-76-9

aldose 1-epimerase; aldose 1-epimerase

Recombinant. From Escherichia coli.
In 3.2 M ammonium sulphate.

For use under Patent No. US 7,785,771 B2 and EP1 828 407 (GB, FR, IE, DE)

Activity/Concentration:
Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25oC.
Galactose mutarotase: 4.1 mg/mL.

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-GALMUT
1 mL; galactose dehydrogenase (200 U/mL) / galactose mutarotase (4 mg/mL)
$260.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

Galactose dehydrogenase - Galactose mutarotase 

EC 1.1.1.48 (Galactose dehydrogenase)
CAS: 9028-54-0

EC 5.1.3.3 (Galactose mutarotase)
CAS: 9031-76-9

Synonyms:
(Galactose dehydrogenase): D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase
(Galactose mutarotase): Aldose 1-epimerase; Aldose 1-epimerase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Activity/Concentration:
Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25oC.
Galactose mutarotase: 4.1 mg/mL.

Unit definition:
One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ per minute at pH 8.6, at 25oC.

Specificity:
Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by galactose mutarotase (GMR) (1).

             (β-galactose mutarotase)
(1) α-D-Galactose  β-D-Galactose

The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of galactose dehydrogenase (GALDH) at pH 7.5 (2).

                       (β-galactose dehydrogenase)
(2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Applications:
Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via d-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR).

Assay for determination of free D-galactose and/or L-arabinose.

Charnock, S. C. & McCleary, B. V. (2006). Irish Patent No. S84280, UK Patent No. 1828407, German Patent No. 1828407, French Patent No. 1828407 and USA Patent No. US7, 785, 771/11/722,000.

Method for the direct determination of available carbohydrates in low-carbohydrate products using high-performance anion exchange chromatography.

Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W. & Ragan, R. (2010). Journal of AOAC International, 93(6), 1897-1904.